Brn-5/BRN5/POU6F1 Antibody (E-30): sc-81982

Brn-5 Antibody (E-30) is a mouse monoclonal IgG2a kappa light chain antibody that detects Brn-5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Brn-5 antibody (E-30) is available as a non-conjugated format. Brn-5, also known as POU6F1, MPOU, or TCFB1, plays a crucial role in the development of the central nervous system, particularly during embryogenesis, where Brn-5 is predominantly expressed in the developing brain and spinal cord. As a member of the Brn family of transcription factors, Brn-5 is characterized by two DNA-binding domains: the POU-specific domain and POU homeodomain, each containing a helix-turn-helix motif that facilitates DNA interaction. This structural feature is essential for function, as Brn-5 binds with high affinity to corticotrphin-releasing hormone elements, thereby enhancing prolactin gene expression and activating Pit-1 expression, which are vital processes in neurodevelopment. Brn-5′s ability to regulate gene expression underscores importance in neuronal differentiation and overall development of the central nervous system, making anti-Brn-5 antibody (E-30) a valuable tool for researchers studying these critical biological processes.

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Brn-5/BRN5/POU6F1 Antibody (E-30) References:

1. The POU gene Brn-5 is induced by neuregulin and is restricted to myelinating Schwann cells.  |  Wu, R., et al. 2001. Mol Cell Neurosci. 17: 683-95. PMID: 11312604
2. Involvement of mPOU (Brn-5), a class VI POU protein, in the gene expression of Pit-1 as well as PRL.  |  Toda, K., et al. 2008. Mol Cell Endocrinol. 280: 20-9. PMID: 17933456
3. Crystallization and preliminary X-ray analysis of human Brn-5 transcription factor in complex with DNA.  |  Pereira, JH., et al. 2008. Acta Crystallogr Sect F Struct Biol Cryst Commun. 64: 175-8. PMID: 18323601
4. Brn-5 is a divergent POU domain factor highly expressed in layer IV of the neocortex.  |  Andersen, B., et al. 1993. J Biol Chem. 268: 23390-8. PMID: 7901208
5. POU domain factors of the Brn-3 class recognize functional DNA elements which are distinctive, symmetrical, and highly conserved in evolution.  |  Gruber, CA., et al. 1997. Mol Cell Biol. 17: 2391-400. PMID: 9111308
6. POU domain genes are differentially expressed in the early stages after lineage commitment of the PNS-derived stem cell line, RT4-AC.  |  Donahue, LM. and Reinhart, AJ. 1998. Brain Res Dev Brain Res. 106: 1-12. PMID: 9554933
7. Potassium chloride inhibits proliferation of cerebellar granule neuron progenitors.  |  Cui, H. and Bulleit, RF. 1998. Brain Res Dev Brain Res. 106: 129-35. PMID: 9554981
8. Expression of the POU transcription factor Brn-5 is an early event in the terminal differentiation of CNS neurons.  |  Cui, H. and Bulleit, RF. 1998. J Neurosci Res. 52: 625-32. PMID: 9669311
9. Highly cooperative homodimerization is a conserved property of neural POU proteins.  |  Rhee, JM., et al. 1998. J Biol Chem. 273: 34196-205. PMID: 9852081

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Brn-5/BRN5/POU6F1 Antibody (E-30) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Brn-5/BRN5/POU6F1 Antibody (E-30) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Brn-5/BRN5/POU6F1 Antibody (E-30) Specifications

Alternate Product Name: Brn-5

Product Clone Name: E-30

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 193-302

Epitope Target: Brn-5

Epitope Species: human

Product Reactivity/Detection: Brn-5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Brn-5/BRN5/POU6F1 Antibody (E-30): N/A