Brn-3c/BRN3C/POU4F3 Antibody (QQ8): sc-81980

Brn-3c Antibody (QQ8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Brn-3c protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Brn-3c antibody (QQ8) is available as the non-conjugated form. Brn-3c, a member of the Brn family of transcription factors, plays a crucial role in the development of the central nervous system, particularly in the differentiation and survival of specific neuronal populations. Located predominantly in the nucleus, Brn-3c is essential for the transcriptional regulation of genes involved in neuronal identity and function. Brn-3c′s ability to bind to specific DNA sequences allows activation or repression of target genes, thereby influencing neuronal development and plasticity. Notably, Brn-3c is part of a larger family that includes Brn-3a and Brn-3b, with Brn-3a functioning as a transcriptional activator and Brn-3b acting primarily as a repressor. The interplay between these proteins is vital for maintaining the balance of gene expression necessary for proper neuronal function. Brn-3c′s unique structural features, including POU domain, facilitate interaction with other transcription factors and regulatory proteins, underscoring Brn-3c′s importance in the intricate network of gene regulation during neurogenesis.

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Brn-3c/BRN3C/POU4F3 Antibody (QQ8) References:

1. A novel POU homeodomain gene specifically expressed in cells of the developing mammalian nervous system.  |  Collum, RG., et al. 1992. Nucleic Acids Res. 20: 4919-25. PMID: 1357630
2. Brn-3c (POU4F3) regulates BDNF and NT-3 promoter activity.  |  Clough, RL., et al. 2004. Biochem Biophys Res Commun. 324: 372-81. PMID: 15465029
3. The neuronal nicotinic acetylcholine receptor alpha 2 subunit gene promoter is activated by the Brn-3b POU family transcription factor and not by Brn-3a or Brn-3c.  |  Milton, NG., et al. 1995. J Biol Chem. 270: 15143-7. PMID: 7797498
4. Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2.  |  Schonemann, MD., et al. 1995. Genes Dev. 9: 3122-35. PMID: 8543156
5. Preferential differentiation of P19 mouse embryonal carcinoma cells into smooth muscle cells. Use of retinoic acid and antisense against the central nervous system-specific POU transcription factor Brn-2.  |  Suzuki, T., et al. 1996. Circ Res. 78: 395-404. PMID: 8593698
6. The different activities of the two activation domains of the Brn-3a transcription factor are dependent on the context of the binding site.  |  Budhram-Mahadeo, V., et al. 1996. J Biol Chem. 271: 9108-13. PMID: 8621561
7. POU domain factor Brn-3b is required for the development of a large set of retinal ganglion cells.  |  Gan, L., et al. 1996. Proc Natl Acad Sci U S A. 93: 3920-5. PMID: 8632990
8. Role of transcription factors Brn-3.1 and Brn-3.2 in auditory and visual system development.  |  Erkman, L., et al. 1996. Nature. 381: 603-6. PMID: 8637595
9. Brn-3.0 expression identifies early post-mitotic CNS neurons and sensory neural precursors.  |  Fedtsova, NG. and Turner, EE. 1995. Mech Dev. 53: 291-304. PMID: 8645597
10. A single amino acid change converts an inhibitory transcription factor into an activator.  |  Dawson, SJ., et al. 1996. J Biol Chem. 271: 11631-3. PMID: 8662774
11. Mutation in transcription factor POU4F3 associated with inherited progressive hearing loss in humans.  |  Vahava, O., et al. 1998. Science. 279: 1950-4. PMID: 9506947

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Brn-3c/BRN3C/POU4F3 Antibody (QQ8) has 43 citations in a variety of scientific publications.

Primary antibodies like Anti-Brn-3c/BRN3C/POU4F3 Antibody (QQ8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Brn-3c/BRN3C/POU4F3 Antibody (QQ8) Specifications

Alternate Product Name: POU4F3

Product Clone Name: QQ8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 100-191

Epitope Target: Brn-3c

Epitope Species: human

Product Reactivity/Detection: Brn-3c

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 43 citations in various scientific publications

Available Conjugates/Forms for Brn-3c/BRN3C/POU4F3 Antibody (QQ8): N/A

The preferred Brn-3/BRN3/POU4F antibody is Brn-3/BRN3/POU4F Antibody (A-4): sc-390780.