Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Brn-3b/BRN3B/POU4F2 Lentiviral Activation Particles (h): sc-400382-LAC
- Target species: human
- 200 µl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles
- Brn-3b/BRN3B/POU4F2 Lentiviral Activation Particles (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells
- Brn-3b/BRN3B/POU4F2 Lentiviral Activation Particles (h) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes
- Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Brn-3b/BRN3B/POU4F2 Lentiviral Activation Plasmid (h) and Brn-3b/BRN3B/POU4F2 Lentiviral Activation Plasmid (h2) target distinct regulatory regions of the POU4F2 promoter. One or both designs may be available
- Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: Brn-3b/BRN3B/POU4F2 Antibody (D-8): sc-514474
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Brn-3b/BRN3B/POU4F2 Lentiviral Activation Particles (h) | sc-400382-LAC | 200 µl | $455.00 |
POU4F2 (Brn-3b/BRN3B) is a POU-homeodomain transcription factor that controls cell type–specific gene expression programs, with prominent roles in neuronal development, differentiation, and survival. By binding regulatory DNA motifs and coordinating transcriptional networks, BRN3B influences processes such as axon guidance, synaptic maturation, and stress-responsive transcription. In human systems, altered POU4F2 activity is used to interrogate mechanisms of neurodevelopmental regulation and lineage specification, and its downstream targets connect to pathways governing apoptosis, cell-cycle control, and neuronal identity. These features make POU4F2 a useful node for studying transcriptional circuitry in neural models and disease-relevant perturbations affecting neuronal fate and resilience.
Brn-3b/BRN3B/POU4F2 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient POU4F2 upregulation across a broader range of human cell types.
Brn-3b/BRN3B/POU4F2 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the POU4F2 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous Brn-3b/BRN3B/POU4F2 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native POU4F2 genomic locus and regulatory architecture.
The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.