BRIP1 Antibody (E-11): sc-365708
- BRIP1 Antibody (E-11) is a mouse monoclonal IgG1 κ BRIP1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 653-691 mapping within an internal region of BRIP1 of human origin
- BRIP1 Antibody (E-11) is recommended for detection of BRIP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-BRIP1 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for BRIP1 Antibody (E-11) for WB and IHC(P) applications. This reagent is now offered in a bundle with BRIP1 Antibody (E-11) (see ordering information below).
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BRIP1 Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects BRIP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-BRIP1 antibody (E-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BRIP1, also known as BACH1 or BRCA1-associated carboxy-terminal helicase-1, plays a crucial role in maintaining genomic stability by participating in DNA repair processes. Specifically, BRIP1 is essential for resolving secondary structures of guanine-rich DNA that can form during lagging-strand DNA synthesis, which is vital for preventing genomic instability that can lead to tumorigenesis. BRIP1 binds directly to the BRCT repeats of BRCA1, and this complex formation is critical for BRCA1′s function in DNA damage response. Phosphorylation of BRIP1 at Ser 990 is required for BRCA1 interaction, linking BRIP1′s activity to the DNA damage-induced checkpoint control during the G2 to M phase transition of the cell cycle. With ubiquitous expression, particularly in the testis, BRIP1 functions as a key player in the caretaker class of genes that safeguard genome integrity, making BRIP1 (E-11) monoclonal antibody an invaluable tool for researchers studying DNA repair mechanisms and their implications in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BRIP1 Antibody (E-11) References:
- BACH1, a novel helicase-like protein, interacts directly with BRCA1 and contributes to its DNA repair function. | Cantor, SB., et al. 2001. Cell. 105: 149-60. PMID: 11301010
- Distinct functions of BRCA1 and BRCA2 in double-strand break repair. | Liu, Y. and West, SC. 2002. Breast Cancer Res. 4: 9-13. PMID: 11879553
- The BRCT domain is a phospho-protein binding domain. | Yu, X., et al. 2003. Science. 302: 639-42. PMID: 14576433
- Phosphopeptide binding specificities of BRCA1 COOH-terminal (BRCT) domains. | Rodriguez, M., et al. 2003. J Biol Chem. 278: 52914-8. PMID: 14578343
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRIP1 Antibody (E-11) | sc-365708 | 200 µg/ml | $322.00 | |||
BRIP1 Antibody (E-11): m-IgG1 BP-HRP Bundle | sc-531708 | 200 µg Ab; 20 µg BP | $361.00 | |||
BRIP1 Antibody (E-11) AC | sc-365708 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BRIP1 Antibody (E-11) HRP | sc-365708 HRP | 200 µg/ml | $322.00 | |||
BRIP1 Antibody (E-11) FITC | sc-365708 FITC | 200 µg/ml | $336.00 | |||
BRIP1 Antibody (E-11) PE | sc-365708 PE | 200 µg/ml | $349.00 | |||
BRIP1 Antibody (E-11) Alexa Fluor® 488 | sc-365708 AF488 | 200 µg/ml | $364.00 | |||
BRIP1 Antibody (E-11) Alexa Fluor® 546 | sc-365708 AF546 | 200 µg/ml | $364.00 | |||
BRIP1 Antibody (E-11) Alexa Fluor® 594 | sc-365708 AF594 | 200 µg/ml | $364.00 | |||
BRIP1 Antibody (E-11) Alexa Fluor® 647 | sc-365708 AF647 | 200 µg/ml | $364.00 | |||
BRIP1 Antibody (E-11) Alexa Fluor® 680 | sc-365708 AF680 | 200 µg/ml | $364.00 | |||
BRIP1 Antibody (E-11) Alexa Fluor® 790 | sc-365708 AF790 | 200 µg/ml | $364.00 |