BRF2 Antibody (C-8): sc-390312

BRF2 Antibody (C-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects BRF2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BRF2 (C-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BRF2 plays a crucial role in transcriptional regulation of small nuclear RNAs, particularly in recruiting RNA polymerase III to specific promoters, such as human U6 small nuclear RNA promoter. Unlike BRF1, which forms a stable complex with TATA-binding protein, BRF2 does not create a stable assembly with components, allowing for more dynamic regulation of transcription. This functional distinction is vital for precise control of gene expression, particularly in processes such as cell growth and differentiation, where timely synthesis of small nuclear RNAs is essential. BRF2′s ability to interact with various transcription factors and involvement in transcription machinery underscores BRF2′s importance in cellular function and highlights anti-BRF2 antibody (C-8) relevance in research applications aimed at understanding gene regulation mechanisms.

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BRF2 Antibody (C-8) References:

1. Essential roles of Bdp1, a subunit of RNA polymerase III initiation factor TFIIIB, in transcription and tRNA processing.  |  Ishiguro, A., et al. 2002. Mol Cell Biol. 22: 3264-75. PMID: 11971960
2. The fission yeast TFIIB-related factor limits RNA polymerase III to a TATA-dependent pathway of TBP recruitment.  |  Huang, Y., et al. 2003. Nucleic Acids Res. 31: 2108-16. PMID: 12682361
3. CK2 phosphorylation of Bdp1 executes cell cycle-specific RNA polymerase III transcription repression.  |  Hu, P., et al. 2004. Mol Cell. 16: 81-92. PMID: 15469824
4. Human Maf1 negatively regulates RNA polymerase III transcription via the TFIIB family members Brf1 and Brf2.  |  Rollins, J., et al. 2007. Int J Biol Sci. 3: 292-302. PMID: 17505538
5. Differential expression of the TFIIIB subunits Brf1 and Brf2 in cancer cells.  |  Cabarcas, S., et al. 2008. BMC Mol Biol. 9: 74. PMID: 18700021
6. The symmetry of the yeast U6 RNA gene's TATA box and the orientation of the TATA-binding protein in yeast TFIIIB.  |  Whitehall, SK., et al. 1995. Genes Dev. 9: 2974-85. PMID: 7498793
7. Cloning, expression, and function of TFC5, the gene encoding the B' component of the Saccharomyces cerevisiae RNA polymerase III transcription factor TFIIIB.  |  Kassavetis, GA., et al. 1995. Proc Natl Acad Sci U S A. 92: 9786-90. PMID: 7568218
8. Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high-mobility-group protein 2-related domains.  |  Wang, Z. and Roeder, RG. 1995. Proc Natl Acad Sci U S A. 92: 7026-30. PMID: 7624363

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BRF2 Antibody (C-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-BRF2 Antibody (C-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BRF2 Antibody (C-8) Specifications

Alternate Product Name: BRF2

Product Clone Name: C-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 19-318

Epitope Target: BRF2

Epitope Species: human

Product Reactivity/Detection: BRF2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for BRF2 Antibody (C-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin