BrdUTP (CAS 102212-99-7)

BrdUTP, scientifically known as 5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt, is a well-established nucleoside analog utilized in scientific research for many years. Acting as a substitute for thymidine, this compound becomes a part of DNA during replication, resulting in the creation of a modified DNA strand. Molecular biologists have extensively employed BrdUTP for various purposes, such as investigating cell proliferation, DNA labeling, and detecting DNA damage. Its applications include assessing DNA synthesis in cells, utilizing specific antibodies or fluorescent probes for detection. Additionally, BrdUTP has proved valuable in studying the impact of different drugs and chemicals on DNA replication and cell proliferation. Notably, BrdUTP′s incorporation during replication replaces thymidine in the newly synthesized strand, leading to the formation of a modified DNA strand that can be identified using specific antibodies or fluorescent probes. It is worth noting that this incorporation can also result in DNA damage, as the modified DNA strand may not be recognized by the DNA repair machinery.