BRCC3 Antibody (AT3B1): sc-517392

BRCC3 Antibody (AT3B1) is a mouse monoclonal IgG1 kappa light chain antibody that detects BRCC3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-BRCC3 antibody (AT3B1) is available as a non-conjugated monoclonal isotype antibody. BRCC3, also known as C6.1A or BRISC complex subunit BRCC36, is a crucial 316 amino acid nuclear protein that functions as a ubiquitin E3 ligase, playing a significant role in the cellular response to DNA damage. Specifically, BRCC3 localizes to sites of double-strand DNA breaks, where BRCC3 acts as a metalloprotease, cleaving Lys-6-linked polyubiquitin chains to promote cellular survival and facilitate DNA repair processes. This function is vital for maintaining genomic stability, particularly in cells undergoing replication stress or damage. BRCC3 exists in five alternatively spliced isoforms and is expressed in various tissues, including skeletal muscle, pancreas, kidney, heart, placenta, liver, lung, and brain. BRCC3 is a member of the peptidase M67A family and the BRCC36 subfamily, and BRCC3 gene is located on human chromosome Xq28 and mouse chromosome X A7.3. Chromosome X is notable for its association with several genetic disorders, such as color blindness, hemophilia, and Duchenne muscular dystrophy, which predominantly affect males due to their single X chromosome.

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BRCC3 Antibody (AT3B1) References:

1. Differential divergence of three human pseudoautosomal genes and their mouse homologs: implications for sex chromosome evolution.  |  Gianfrancesco, F., et al. 2001. Genome Res. 11: 2095-100. PMID: 11731500
2. Overall DNA methylation and chromatin structure of normal and abnormal X chromosomes.  |  Bernardino-Sgherri, J., et al. 2002. Cytogenet Genome Res. 99: 85-91. PMID: 12900549
3. Isolation and sequence of two genes associated with a CpG island 5' of the factor VIII gene.  |  Kenwrick, S., et al. 1992. Hum Mol Genet. 1: 179-86. PMID: 1303175
4. Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalosome-like subunit and its role in DNA repair.  |  Dong, Y., et al. 2003. Mol Cell. 12: 1087-99. PMID: 14636569
5. The molecular basis of variation in human color vision.  |  Deeb, SS. 2005. Clin Genet. 67: 369-77. PMID: 15811001
6. Expression of BRCC3, a novel cell cycle regulated molecule, is associated with increased phospho-ERK and cell proliferation.  |  Boudreau, HE., et al. 2007. Int J Mol Med. 19: 29-39. PMID: 17143545
7. The BRCC3 regulated by Cdk5 promotes the activation of neuronal NLRP3 inflammasome in Parkinson's disease models.  |  Cheng, X., et al. 2020. Biochem Biophys Res Commun. 522: 647-654. PMID: 31787240
8. BRCC3 promotes activation of the NLRP6 inflammasome following cerebral ischemia/reperfusion (I/R) injury in rats.  |  Huang, X., et al. 2021. Neurosci Lett. 756: 135954. PMID: 33979701
9. BRCC3 Promotes Tumorigenesis of Bladder Cancer by Activating the NF-κB Signaling Pathway Through Targeting TRAF2.  |  Tao, H., et al. 2021. Front Cell Dev Biol. 9: 720349. PMID: 34604222
10. BRCC3-Mediated NLRP3 Deubiquitylation Promotes Inflammasome Activation and Atherosclerosis in Tet2 Clonal Hematopoiesis.  |  Yalcinkaya, M., et al. 2023. Circulation. 148: 1764-1777. PMID: 37781816
11. BRCC3 mediates inflammation and pyroptosis in cerebral ischemia/reperfusion injury by activating the NLRP6 inflammasome.  |  Huang, X., et al. 2024. CNS Neurosci Ther. 30: e14697. PMID: 38544474
12. BRCC3 Regulation of ALK2 in Vascular Smooth Muscle Cells: Implication in Pulmonary Hypertension.  |  Shen, H., et al. 2024. Circulation. 150: 132-150. PMID: 38557054

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BRCC3 Antibody (AT3B1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-BRCC3 Antibody (AT3B1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BRCC3 Antibody (AT3B1) Specifications

Alternate Product Name: BRCC3

Product Clone Name: AT3B1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-316

Epitope Target: BRCC3

Epitope Species: human

Product Reactivity/Detection: BRCC3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide, 0.1% gelatin and 1% glycerol

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for BRCC3 Antibody (AT3B1): N/A