BRCA2 Double Nickase Plasmid (h): sc-400700-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BRCA2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BRCA2 Double Nickase Plasmid (h) and BRCA2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BRCA2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BRCA2 Antibody (D-8): sc-518154
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRCA2 Double Nickase Plasmid (h) | sc-400700-NIC | 20 µg | $410.00 | |||
BRCA2 Double Nickase Plasmid (h2) | sc-400700-NIC-2 | 20 µg | $410.00 |
BRCA2 encodes a nuclear tumor suppressor that orchestrates high-fidelity DNA double-strand break repair by homologous recombination through regulation of RAD51 filament loading and stabilization at resected DNA ends. It functions at stalled replication forks to protect nascent DNA and support fork restart, linking BRCA2 activity to replication stress responses and genome integrity checkpoints. Disruption of BRCA2 leads to chromosomal instability and hypersensitivity to DNA-damaging stress, making it a central node in DNA repair pathway studies. Genetic alterations in BRCA2 are strongly associated with hereditary and sporadic cancer susceptibility, and BRCA2 status is widely used in research to interrogate homologous recombination deficiency and DNA damage signaling.
BRCA2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BRCA2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BRCA2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BRCA2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BRCA2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.