BRCA1 Double Nickase Plasmid (h): sc-400093-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BRCA1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BRCA1 Double Nickase Plasmid (h) and BRCA1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BRCA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BRCA1 Antibody (D-9): sc-6954
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRCA1 Double Nickase Plasmid (h) | sc-400093-NIC | 20 µg | $410.00 | |||
BRCA1 Double Nickase Plasmid (h2) | sc-400093-NIC-2 | 20 µg | $410.00 |
BRCA1 encodes a tumor suppressor protein that functions as a central scaffold in the DNA damage response, coordinating homologous recombination repair, replication fork protection, and cell-cycle checkpoint control. BRCA1 forms multiprotein complexes that regulate double-strand break processing and chromatin-associated signaling, including interactions with PALB2/BRCA2 and ATM/ATR-mediated phosphorylation networks. Disruption of BRCA1 compromises genome stability, increasing reliance on error-prone repair pathways and promoting accumulation of chromosomal abnormalities. BRCA1 dysfunction is strongly linked to hereditary breast and ovarian cancer predisposition and is widely studied as a determinant of DNA repair capacity and replication stress sensitivity.
BRCA1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BRCA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BRCA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BRCA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BRCA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.