Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
bradykinin B1 R Double Nickase Plasmid (h): sc-401169-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- bradykinin B1 R Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- bradykinin B1 R Double Nickase Plasmid (h) and bradykinin B1 R Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BDKRB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: bradykinin B1 R Antibody (F-11): sc-518136
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
bradykinin B1 R Double Nickase Plasmid (h) | sc-401169-NIC | 20 µg | $410.00 | |||
bradykinin B1 R Double Nickase Plasmid (h2) | sc-401169-NIC-2 | 20 µg | $410.00 |
BDKRB1 encodes the inducible bradykinin B1 receptor, a G protein–coupled receptor upregulated by inflammatory cues and tissue injury. Upon activation by des-Arg kinins, bradykinin B1 R promotes signaling through Gαq/Gαi pathways that elevate intracellular calcium and stimulate MAPK and NF-κB programs, shaping cytokine production, leukocyte recruitment, and vascular responses. This receptor participates in inflammatory and nociceptive signaling networks and is frequently studied in contexts such as chronic inflammation and pain-related mechanisms, as well as vascular dysfunction and metabolic stress. Altered BDKRB1 expression and signaling has been associated with disease-relevant inflammatory microenvironments, supporting its use as a mechanistic node for pathway dissection in human cell models.
bradykinin B1 R Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BDKRB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BDKRB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BDKRB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BDKRB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.