BoNT/E Antibody (11D4): sc-80470

BoNT/E Antibody (11D4) is a mouse monoclonal IgG1 antibody that detects Botulinum neurotoxin type E in Clostridium botulinum by enzyme-linked immunosorbent assay (ELISA). BoNT/E is a potent neurotoxin that plays a critical role in the pathogenesis of botulism, a serious paralytic illness. Anti-BoNT/E antibody (11D4) functions by recognizing the neurotoxin that cleaves synaptosomal-associated protein 25 (SNAP-25), a key component of the synaptic vesicle fusion machinery essential for neurotransmitter release such as acetylcholine. The inhibition of SNAP-25 leads to a significant reduction in acetylcholine release at the neuromuscular junction, resulting in flaccid paralysis. Understanding the structure of BoNT/E is crucial, as this neurotoxin consists of a heavy chain and a light chain connected by a disulfide bond, which facilitates entry into neurons and subsequent enzymatic activity. The structural integrity of BoNT/E is vital for neurotoxic effects, as any alterations could impact binding to neuronal receptors and disrupt neurotransmitter release. BoNT/E monoclonal antibody (11D4) serves as an invaluable tool for researchers studying the mechanisms of botulism and the therapeutic potential of targeting this neurotoxin in various neurological disorders.