BoNT/D Antibody (5131): sc-57637

BoNT/D Antibody (5131) is a mouse monoclonal IgG1 antibody that detects the BoNT/D protein of C. botulinum origin by western blotting (WB) and immunofluorescence (IF). Anti-BoNT/D antibody (5131) is available as the non-conjugated format, providing researchers with a reliable tool for studying this critical neurotoxin. Botulism, a rare yet severe paralytic illness, is caused by the potent nerve toxin produced by the anaerobic bacterium Clostridium botulinum. Understanding the function of BoNT/D protein is essential, as BoNT/D protein plays a pivotal role in the pathogenesis of botulism by cleaving VAMP-2, a key component of the SNARE complex necessary for synaptic vesicle fusion. This cleavage disrupts the release of acetylcholine, leading to flaccid paralysis, which underscores the importance of BoNT/D in neuromuscular transmission. Clostridium botulinum serotype D strains typically produce two stable toxin complexes: the M toxin, which consists of neurotoxin and nontoxic-nonhemagglutinin, and the L toxin, which includes the M toxin along with hemagglutinin subcomponents. The interaction between BoNT/D and VAMP-2 is facilitated by a hydrophobic interaction with the V1 motif during the cleavage process, highlighting the intricate molecular mechanisms that underlie the neurotoxic effects of this pathogen.

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BoNT/D Antibody (5131) References:

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