BMP-8B Double Nickase Plasmid (h): sc-404893-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BMP-8B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BMP-8B Double Nickase Plasmid (h) and BMP-8B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BMP8B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BMP-8B Antibody (AT13E6): sc-517391
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMP-8B Double Nickase Plasmid (h) | sc-404893-NIC | 20 µg | $410.00 | |||
BMP-8B Double Nickase Plasmid (h2) | sc-404893-NIC-2 | 20 µg | $410.00 |
BMP8B encodes bone morphogenetic protein 8B (BMP-8B), a secreted ligand in the TGF-β/BMP superfamily that signals through type I/II serine/threonine kinase receptors to activate SMAD1/5/9-dependent transcriptional programs. BMP-8B contributes to regulation of developmental patterning, tissue differentiation, and extracellular matrix–associated processes, with downstream effects on cell fate decisions and morphogenesis. In adult physiology, BMP pathway activity involving BMP8B has been linked to metabolic regulation and thermogenic adipose biology, while broader BMP signaling dysregulation is associated with fibrosis, skeletal abnormalities, and cancer-related phenotypes. As such, BMP8B is frequently studied to dissect context-specific BMP signaling dynamics, receptor utilization, and pathway crosstalk with MAPK/PI3K networks in human cells.
BMP-8B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BMP8B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BMP8B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BMP8B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BMP8B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.