BMP-1 Double Nickase Plasmid (h): sc-402432-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BMP-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BMP-1 Double Nickase Plasmid (h) and BMP-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BMP1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMP-1 Double Nickase Plasmid (h) | sc-402432-NIC | 20 µg | $410.00 | |||
BMP-1 Double Nickase Plasmid (h2) | sc-402432-NIC-2 | 20 µg | $410.00 |
BMP1 encodes bone morphogenetic protein 1 (BMP-1), a secreted metalloprotease of the astacin family that functions as a procollagen C-proteinase and regulator of extracellular matrix maturation. BMP-1 cleaves multiple matrix precursors and modulators, including fibrillar procollagens and proteins that influence TGF-β/BMP signaling, thereby coordinating collagen fibrillogenesis, tissue remodeling, and morphogenesis. Through these activities, BMP-1 impacts pathways linked to matrix deposition, fibrosis, and wound repair, and altered BMP1 function has been associated with heritable connective tissue and bone fragility phenotypes. BMP1 is therefore widely studied in cell and tissue models to dissect ECM-dependent signaling, stromal remodeling, and matrix-driven changes in cell behavior.
BMP-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BMP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BMP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BMP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BMP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.