Blood Group Lewis a Antibody (7LE) recommended for detection of Lewis a blood group antigen of human origin by WB, IP, IF and IHC(P)

Blood Group Lewis a Antibody (7LE): sc-51512

Blood Group Lewis a Antibody (7LE) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-Blood Group Lewis a Antibody (7LE) is a mouse monoclonal IgG1 (kappa light chain) Blood Group Lewis a antibody provided at 200 µg/ml
    • raised against Mucin isolated from human ovarian cyst fluid
    • Blood Group Lewis a Antibody (7LE) is recommended for detection of Lewis a blood group antigen of human origin by WB, IP, IF and IHC(P)
    • Anti-Blood Group Lewis a Antibody (7LE) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Blood Group Lewis a (7LE): sc-51512.

I am using Blood Group Lewis a (7LE): sc-51512 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong Worked great in IF at 1:50 dilution, staining data very strong
Date published: 2017-05-09
Rated 5 out of 5 by from Great Data! This antibody is recommended for detection of Lewis a blood group antigen of human origin by WB, IP, IF and IHC(P).
Date published: 2017-02-07
Rated 5 out of 5 by from Lots of signal detected in western blot analysis Lots of signal detected in western blot analysis of Blood Group Lewis a expression in WiDr whole cell lysate. -SCBT QC
Date published: 2013-07-23
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