Blasticidin S Hydrochloride CAS: 3513-03-9
MF: C17H26N8O5•HCl
MW: 458.9

Blasticidin S Hydrochloride (CAS 3513-03-9)

Blasticidin S Hydrochloride | CAS 3513-03-9 is rated 5.0 out of 5 by 2.
  • y_2020, m_5, d_28, h_14
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.8
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_204655, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 183ms
  • REVIEWS, PRODUCT
5
2
4
0
3
0
2
0
1
0
Alternate Names: Blasticidin S Hydrochloride also known as BLASTICIDIN S HCL; BLASTICIDIN S HYDROCHLORIDE; BLASTICIDIN S, HYDROCHLORIDE, STREPTOMYCES; GRISEOCHROMOGENES; blasticidin S
Application: Blasticidin S Hydrochloride is an antibiotic inhibiting protein synthesis and belongs to a group of cytosine amino nucleoside antibiotics
CAS Number: 3513-03-9
Purity: ≥98%
Molecular Weight: 458.9
Molecular Formula: C17H26N8O5•HCl
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Blasticidin S Hydrochloride, like puromycin (sc-205821), belongs to a group of cytosine amino nucleoside antibiotics. Blasticidin S is isolated from Streptomyces griseochromogenes and inhibits protein synthesis in both prokaryotic and eukaryotic cells, Blasticidin S Hydrochloride acts quickly by inhibiting peptide bond formation by the ribosome.

The blasticidin S resistance gene (BSR), found in Bacillus cereus K55-S1, has been used in multiple studies for its use as a selective compound. Resistance is conferred by expression of one of two Blasticidin S deaminase genes: BSD from Aspergillus terreus or bsr from Bacillus cereus. These deaminases convert Blasticidin S to a nontoxic deaminohydroxy derivative. Blasticidin S Hydrochloride is used as a selection agent for transformed cells that contain the resistance genes bls, bsr or BSD.


References

1. Takeuchi, S., et al. 1958. J. Antibiot. 11: 1-5. PMID: 13525246

2. Swaminathan, V., et al. 1981. Biochim. Biophys. Acta. 655: 335-341. PMID: 7284390

3. Itaya, M., et al. 1990. J. Biochem. 107: 799-801. PMID: 2118136

4. Kamakura, T., et al. 1990. Mol. Gen. Genet. 223: 332-334. PMID: 2250657

5. Izumi, M., et al. 1991. Exp. Cell Res. 197: 229-233. PMID: 1720391

6. Kimura, M., et al. 1994. Biochim. Biophys. Acta. 1219: 653-659. PMID: 7948022

7. Kimura, M., et al. 1994. Mol. Gen. Genet. 242: 121-129. PMID: 8159161

8. Nishimura, M., et al. 2006. J. Biosci. Bioeng. 101: 63-69. PMID: 16503293

Usage :
Blasticidin S is used for the prevention of contamination of cell cultures. The recommended working concentration ranges from 1–20 µg/mL depending on the cell line.


Blasticidin S may also be used as a bacterial selection antibiotic in the concentration range of 50–100 µg/mL. To maintain the activity of Blasticidin S for selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0.

Appearance :
Powder
Physical State :
Solid
Derived From :
Streptomyces girseochromogenes
Solubility :
Soluble in water. Insoluble in organic solvents.
Storage :
Store at 4° C
Melting Point :
218.0-225.0° C
Boiling Point :
772.1° C at 760 mmHg (Predicted)
Refractive Index :
n20D ~1.71 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
25078183
Merck Index :
14: 1316
MDL Number :
MFCD02091640
EC Number :
264-899-3
SMILES :
CN(CC[[email protected]](CC(=O)N[[email protected]]1C=C[[email protected]@H](O[[email protected]@H]1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

Blasticidin S Hydrochloride (CAS 3513-03-9) Product Citations

See how others have used Blasticidin S Hydrochloride (CAS 3513-03-9). Click on the entry to view the PubMed entry .

Citations 1 to 9 of 9 total

PMID: # 30293783  Juszkiewicz, S.|Chandrasekaran, V.|Lin, Z.|Kraatz, S.|Ramakrishnan, V.|Hegde, RS.| et al. 2018. Mol. Cell. 72: 469-481.e7.

PMID: # 28864500  Liu, Y. et al. 2017. Am. J. Physiol. Gastrointest. Liver Physiol. 313: G478-G491.

PMID: # 10744730  Juszkiewicz, S. et al. 2016. J. Biol. Chem. 275: 10413-20.

PMID: # 26391343  Granato, M. et al. 2015. Autophagy. 11: 1978-1986.

PMID: # 26217182  Adragna, NC. et al. 2015. Frontiers in cellular neuroscience. 9: 255.

PMID: # 16476734  Maeda, K. et al. 2006. J. Biol. Chem. 281: 12688-12698.

PMID: # 32187542  Cell Rep. 30: 3691-3698.e5.

PMID: # 32217696  Cancer Res.

PMID: # 27698002  Clin. Cancer Res. 23: 636-648.

Citations 1 to 9 of 9 total
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 131ms
  • QUESTIONS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Rated 5 out of 5 by from Good! We were very pleased with the antibiotic we purchased.
Date published: 2019-10-08
Rated 5 out of 5 by from This chemical has been working well This chemical has been working well. Very satisfied with purity.
Date published: 2015-04-08
  • y_2020, m_5, d_28, h_14
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.8
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_204655, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 11ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.