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Blasticidin S HCl solution is supplied ready to use at 10 mg/mL in HEPES Buffer and Sterile Filtered. Blasticidin S is a nucleoside antibiotic isolated from Streptomyces griseochromogenes which inhibits protein synthesis in both prokaryotic and eukaryotic cells. Blasticidin S acts quickly by inhibiting peptide bond formation by the ribosome. Resistance is conferred by expression of one of two Blasticidin S deaminase genes: BSD from Aspergillus terreus or bsr from Bacillus cereus. These deaminases convert Blasticidin S to a nontoxic deaminohydroxy derivative.
Blasticidin S is used for the prevention of contamination of cell cultures. The recommended working concentration ranges from 1–20 µg/mL depending on the cell line.
Blasticidin S may also be used as a bacterial selection antibiotic in the concentration range of 50–100 µg/mL. To maintain the activity of Blasticidin S for selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0.
1. Yamaguchi, H., Yamamoto, C. and Tanaka, N. 1965. Inhibition of protein synthesis by blasticidin S. I. Studies with cell-free systems from bacterial and mammalian cells. J. Biochem. 57(5): 667-677. PMID: 4953866
2. Izumi, M., Miyazawa, H., Kamakura, T., Yamaguchi, I., Endo, T. and Hanaoka, F. 1991. Blasticidin S-resistance gene (bsr): a novel selectable marker for mammalian cells. Exp. Cell Res. 197(2): 229-233. PMID: 1720391
3. Kimura, M., Takatsuki, A. and Yamaguchi, I. 1994. Blasticidin S deaminase gene from Aspergillus terreus (BSD): a new drug resistance gene for transfection of mammalian cells. Biochim. Biophys. Acta. 1219(3): 653-659. PMID: 7948022
4. Kim, H., Ishidate, T., Ghanta, K.S., Seth, M., Conte, D., Shirayama, M. and Mello, C.C. 2014. A co-CRISPR strategy for efficient genome editing in Caenorhabditis elegans. Genetics. 197(4): 1069-1080. PMID: 24879462
See how others have used Blasticidin S HCl solution. Click on the entry to view the PubMed entry .
PMID: # 30838870 Wu, M.|Feng, Y.|Ye, GX.|Han, YC.|Wang, SS.|Ni, HF.|Wang, FM.|Gao, M.|Lv, LL.|Liu, BC.| et al. 2019. Am. J. Physiol. Renal Physiol. 316: F1006-F1015.
PMID: # 30881116 Chiang, KC.|Huang, ST.|Wu, RC.|Huang, SC.|Yeh, TS.|Chen, MH.|Hsu, JT.|Chen, LW.|Kuo, SF.|Chueh, HY.|Juang, HH.|Hung, SI.|Yeh, CN.|Pang, JS.| et al. 2019. Cancer Manag Res. 11: 1893-1905.
PMID: # 30034634 Barone, G.|Arora, A.|Ganesh, A.|Abdel-Fatah, T.|Moseley, P.|Ali, R.|Chan, SY.|Savva, C.|Schiavone, K.|Carmell, N.|Myers, KN.|Rakha, EA.|Madhusudan, S.|Collis, SJ.| et al. 2018. Oncotarget. 9: 29508-29524.
PMID: # 30538136 Thwaites, DT.|Carter, C.|Lawless, D.|Savic, S.|Boyes, JM.| et al. 2018. Blood.
PMID: # 31576091 World J. Gastroenterol. 25: 5434-5450.
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