Bisbenzimide H 33258 Fluorochrome, Trihydrochloride CAS: 23491-45-4
MF: C25H24N6O•3HCl
MW: 533.88
An A-T specific highly fluorescent DNA stain that has many uses.

Bisbenzimide H 33258 Fluorochrome, Trihydrochloride (CAS 23491-45-4)

Bisbenzimide H 33258 Fluorochrome, Trihydrochloride | CAS 23491-45-4 is rated 5.0 out of 5 by 1.
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Alternate Names: Hoechst 33258
Application: An A-T specific highly fluorescent DNA stain that has many uses
CAS Number: 23491-45-4
Molecular Weight: 533.88
Molecular Formula: C25H24N6O•3HCl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Bisbenzimide H 33258 Fluorochrome, Trihydrochloride (H 33258) is a membrane-permeable, adenine-thymine-specific fluorescent stain, that is useful for staining DNA, chromosomes, and nuclei. This product has been shown to bind to the minor groove in DNA and can be used to detect mycoplasma contamination in tissue culture, to indicate early onset of apoptosis, and to sort living cells based on DNA content. H 33258 and the related compound H 33342 (sc-200908) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs, and both have been shown to affect the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles; interestingly H 33258 increases eflux while H 33342 (sc-200908) decreases it.


References

1. Latt, S.A., et al. 1976. J. Histochem. Cytochem. 24: 24-33. PMID: 943439
2. Cesarone, C.F., et al. 1979. Anal. Biochem. 100: 188-197. PMID: 94515
3. Jin, R., et al. 1988. Proc. Natl. Acad. Sci. U.S.A. 85: 8939-8942. PMID: 2461559
4. Oberhammer, F., et al. 1993. EMBO J. 12: 3679-3684. PMID: 8253089
5. Papadimitriou, E., et al. 1993. J. Immunol. Methods. 162: 41-45. PMID: 7685366
6. Beeler, T.J., et al. 1993. J. Membr. Biol. 135: 109-118. PMID: 7692064
7. Battaglia, M., et al. 1994. Biotech Histochem. 69: 152-156. PMID: 7520758
8. Elstein, K.H., et al. 1994. Exp. Cell Res. 211: 322-331. PMID: 7511539
9. Tarshis, M., et al. 1994. J. Immunol. Methods. 168: 245-252. PMID: 7508483
10. Werstuck, G., et al. 1998. Science. 282: 296-298. PMID: 9765156

Physical State :
Solid
Solubility :
Soluble in water, water and ethanol (10 mg/ml), and ethanol, precipitates in phosphate buffered solutions
Storage :
Store at 4° C
Fluoresence :
λex 338 nm, λem 505 nm (pH 7.0); λex 355 nm, λem 465 nm in TE buffer; DNA; λex 346 nm, λem 364 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
SM1140500
PubChem CID :
Merck Index :
14: 10367
MDL Number :
MFCD00012679
EC Number :
245-690-6
Beilstein Registry :
4088183
SMILES :
CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)NC(=C6C=CC(=O)C=C6)N5.Cl.Cl.Cl

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Certificate of Analysis

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Bisbenzimide H 33258 Fluorochrome, Trihydrochloride (CAS 23491-45-4)  Product Citations

See how others have used Bisbenzimide H 33258 Fluorochrome, Trihydrochloride (CAS 23491-45-4). Click on the entry to view the PubMed entry .

Citations 1 to 5 of 5 total

PMID: # 16172982  Swetloff, A. et al. 2005. Int. J. Dev. Biol. 49: 859-865.

PMID: # 15837784  Truman, JP. et al. 2005. J. Biol. Chem. 280: 23262-23272.

PMID: # 12952860  Hisayo Fujita et al. 2004. Am. J. Physiol. 286: 120-126.

PMID: # 14608505  Walder, S. et al. 2003. Dev. Genes Evol. 213: 625-630.

PMID: # 9500457  Kondo, S. et al. 1998. Cancer Res. 58: 962-967.

Citations 1 to 5 of 5 total
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Rated 5 out of 5 by from Truman et al Truman et al. (PubMed ID 15837784) used Bisbenzimide H 33258 Fluorochrome, Trihydrochloride, an A-T specific and highly fluorescent DNA stain, to quantify radiation-induced apoptosis of human prostate cancer cells. -SCBT Publication Review
Date published: 2015-04-24
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