Biotin-VAD-FMK is a cell permeable biotin-labeled pan-caspase inhibitor used to identify active caspases in cell lysates. Biotin-VAD-FMK is an irreversible caspase inhibitor that covalently binds to the active cavity of a wide spectrum of active caspases. Affinity labeling of active caspases with biotin-VAD-fmk was carried out under conditions where more than 95% of caspase-3-like activity was inactivated in lysates from TNFα/ActD-treated cells. Biotin-VAD-FMK is an irreversible, pancaspase inhibitor. Inhibits TNFα-mediated apoptosis in vitro (IC50 = 0.7 μM).
1. Thornberry, N. A. and Lazebnik, Y.,1998, Science 281, 1312. PMID: 9721091
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Soluble in high purity DMSO (20 mM).
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Thank you for your question. Each lot of this chemical comes with a lot-specific Certificate Of Analysis which typically describes the percent purity as well as the method used to determine the purity. You may request a lot-specific Certificate Of Analysis on our website. Alternatively, please contact our Technical Service Department to inquire about the purity of our most recent lot.
Answered by: Technical Support
Date published: 2019-01-26
How do you make a stock solution of this product?
Asked by: hawkeye11
Thank you for your question. This chemical, Biotin-VAD-FMK, can be made into a stock solution with high purity DMSO, 20 mM.
Rated 5 out of
Various publications cite the use BiotinVarious publications cite the use Biotin-VAD-FMK, a cell permeable biotin-labeled pan-caspase inhibitor, to identify active caspases in cell lysates. -SCBT Publication Review
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