Biotin-VAD-FMK CAS: 1135688-15-1
MF: C30H49FN6O8S
MW: 672.81

Biotin-VAD-FMK (CAS 1135688-15-1)

Biotin-VAD-FMK | CAS 1135688-15-1 is rated 5.0 out of 5 by 1.
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Alternate Names: Biotin-VAD-FMK also known as Biotin-Val-Ala-Asp(OMe)-Fluoromethyl ketone; pan-caspase inhibitor; Biotin-VAD(OMe)-FMK
Application: Biotin-VAD-FMK is a cell permeable biotin-labeled caspase inhibitor used to identify active caspases in cell lysates
CAS Number: 1135688-15-1
Molecular Weight: 672.81
Molecular Formula: C30H49FN6O8S
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Biotin-VAD-FMK is a cell permeable biotin-labeled pan-caspase inhibitor used to identify active caspases in cell lysates. Biotin-VAD-FMK is an irreversible caspase inhibitor that covalently binds to the active cavity of a wide spectrum of active caspases. Affinity labeling of active caspases with biotin-VAD-fmk was carried out under conditions where more than 95% of caspase-3-like activity was inactivated in lysates from TNFα/ActD-treated cells. Biotin-VAD-FMK is an irreversible, pancaspase inhibitor. Inhibits TNFα-mediated apoptosis in vitro (IC50 = 0.7 μM).


References

1. Thornberry, N. A. and Lazebnik, Y.,1998, Science 281, 1312. PMID: 9721091

2. Li, S., et al., 1999, J. Biol. Chem. 274(24):17325-17333. PMID: 10358093

Physical State :
Solid
Solubility :
Soluble in high purity DMSO (20 mM).
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
25108682
MDL Number :
MFCD01322989
SMILES :
C[[email protected]@H](C(=O)N[[email protected]@H](CC(=O)OC)C(=O)CF)NC(=O)[[email protected]](C(C)C)NC(=O)CCCCCNC(=O)CCCC[[email protected]]1[[email protected]@H]2[[email protected]](CS1)NC(=O)N2

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Certificate of Analysis

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Biotin-VAD-FMK (CAS 1135688-15-1) Product Citations

See how others have used Biotin-VAD-FMK (CAS 1135688-15-1). Click on the entry to view the PubMed entry .

Citations 1 to 3 of 3 total

PMID: # 30232264  Green, JP.|Yu, S.|Martín-Sánchez, F.|Pelegrin, P.|Lopez-Castejon, G.|Lawrence, CB.|Brough, D.| et al. 2018. Proc. Natl. Acad. Sci. U.S.A. 115: E9371-E9380.

PMID: # 27189977  Gibon, J.|Unsain, N.|Gamache, K.|Thomas, RA.|De Leon, A.|Johnstone, A.|Nader, K.|Séguéla, P.|Barker, PA.| et al. 2016. FASEB J. 30: 3083-90.

PMID: # 29791511  PLoS Pathog. 14: e1007105.

Citations 1 to 3 of 3 total

what's the purity of this compound? Thanks

Asked by: li32
Thank you for your question. Each lot of this chemical comes with a lot-specific Certificate Of Analysis which typically describes the percent purity as well as the method used to determine the purity. You may request a lot-specific Certificate Of Analysis on our website. Alternatively, please contact our Technical Service Department to inquire about the purity of our most recent lot.
Answered by: Technical Support
Date published: 2019-01-26

How do you make a stock solution of this product?

Asked by: hawkeye11
Thank you for your question. This chemical, Biotin-VAD-FMK, can be made into a stock solution with high purity DMSO, 20 mM.
Answered by: Chemical Support 1
Date published: 2018-01-04
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Rated 5 out of 5 by from Various publications cite the use Biotin Various publications cite the use Biotin-VAD-FMK, a cell permeable biotin-labeled pan-caspase inhibitor, to identify active caspases in cell lysates. -SCBT Publication Review
Date published: 2015-03-15
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