Biotin PEG2 maleimide
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Biotin PEG2 maleimide is a chemical compound that consists of biotin, a small PEG (polyethylene glycol) spacer (often denoted as PEG2, indicating two repeating units of ethylene glycol), and a maleimide functional group. Biotin is a well-known vitamin that has a high affinity for streptavidin and avidin, which are used in various biological assays and experiments. The PEG2 spacer provides some flexibility and distance between the biotin moiety and the maleimide group. The maleimide functional group is reactive and can conjugate to thiol groups, such as those found on cysteine residues in proteins. Thus, Biotin PEG2 maleimide can be used to label proteins or other molecules containing thiol groups with biotin, allowing for subsequent detection or purification using streptavidin or avidin-based methods. Such methods include immunoprecipitation, enzyme-linked immunosorbent assay (ELISA), western blotting, fluorescence microscopy, flow cytometry, immunohistochemistry, immunocytochemistry, affinity purification, and streptavidin-biotin complex (StABC) amplification.
Biotin PEG2 maleimide References
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- Detection and quantification of free sulfhydryls in monoclonal antibodies using maleimide labeling and mass spectrometry. | Robotham, AC. and Kelly, JF. 2019. MAbs. 11: 757-766. PMID: 30894096
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- Tuning Protein Discrimination Through Altering the Sampling Interface Formed between the Analyte and the OmpG Nanopore. | Fahie, MA., et al. 2021. ACS Sens. 6: 1286-1294. PMID: 33599487
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- Colorimetric allergenic fungal spore detection using peptide-modified gold nanoparticles | Joung In Lee a 1, Seok Cheon Jang a 1, Jinhyo Chung a, Woong-Ku Choi a, Caleb Hong a, Geum Ran Ahn c, Seong Hwan Kim c, Byung Yang Lee d, Woo-Jae Chung a b. 2021. Sensors and Actuators B: Chemical. 327.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Biotin PEG2 maleimide, 5 mg | sc-499382 | 5 mg | $142.00 |