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Biotin LC hydrazide (CAS 109276-34-8)

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Alternate Names:
Biotinamidocaproyl Hydrazide
Application:
Biotin LC hydrazide is carbohydrate reactive with a spacer arm and is more sensitive than biotin hydrazide
CAS Number:
109276-34-8
Molecular Weight:
371.50
Molecular Formula:
C16H29N5O3S
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Biotin LC hydrazide is a chemical reagent used in biochemical and analytical research due to its ability to form stable covalent bonds with aldehyde or ketone groups. This property is harnessed in the labeling and detection of glycoproteins, where the hydrazide functionality reacts specifically with the carbonyl groups of glycosylated proteins. It is also utilized in the immobilization of biomolecules onto surfaces, as the long-chain (LC) spacer increases the molecule′s solubility and accessibility, enhancing its binding efficiency. Additionally, biotin LC hydrazide plays a role in affinity chromatography, where it is used to create biotinylated agarose beads that can be employed to purify or isolate proteins through biotin-streptavidin interactions. In proteomics, it aids in the enrichment and analysis of post-translationally modified proteins, providing insights into protein function and interaction networks within the cell.


Biotin LC hydrazide (CAS 109276-34-8) References

  1. S-Nitrosothiols increases cystic fibrosis transmembrane regulator expression and maturation in the cell surface.  |  Zaman, K., et al. 2014. Biochem Biophys Res Commun. 443: 1257-62. PMID: 24393850
  2. The role of anti-CCD antibodies in grape allergy diagnosis.  |  Falak, R., et al. 2013. Rep Biochem Mol Biol. 1: 74-82. PMID: 26989712
  3. Dataset of KCNQ1, KCNN4, KATP channel expression and dexamethasone modulation of protein kinase signaling in airway epithelial cells.  |  Hynes, D. and Harvey, BJ. 2019. Data Brief. 27: 104642. PMID: 31720320
  4. Validation and evaluation of a common biomarker in human cancers sera protein detected by a monoclonal antibody UNIVmAb.  |  Manjunath, D., et al. 2019. BMC Res Notes. 12: 744. PMID: 31727145
  5. LPS decreases CFTR open probability and mucociliary transport through generation of reactive oxygen species.  |  Cho, DY., et al. 2021. Redox Biol. 43: 101998. PMID: 33971543
  6. Understanding the structure and composition of recalcitrant oligosaccharides in hydrolysate using high-throughput biotin-based glycome profiling and mass spectrometry.  |  Xue, S., et al. 2022. Sci Rep. 12: 2521. PMID: 35169269
  7. Ai-Tong-An-Gao-Ji and Fisetin Inhibit Tumor Cell Growth in Rat CIBP Models by Inhibiting the AKT/HIF-1α Signaling Pathway.  |  Wang, J., et al. 2022. J Oncol. 2022: 1459636. PMID: 35222641
  8. Molecular Mechanism Investigation on Monomer Kaempferol of the Traditional Medicine Dingqing Tablet in Promoting Apoptosis of Acute Myeloid Leukemia HL-60 Cells.  |  Zheng, D., et al. 2022. Evid Based Complement Alternat Med. 2022: 8383315. PMID: 35251215
  9. Inhibitory Potential of Shen-Shuai-Ling Formulation on Renal Interstitial Fibrosis via Upregulation of PLZF.  |  Song, N., et al. 2022. Evid Based Complement Alternat Med. 2022: 5967804. PMID: 35399631
  10. Sulfated Hyaluronan Binds to Heparanase and Blocks Its Enzymatic and Cellular Actions in Carcinoma Cells.  |  Shi, J., et al. 2022. Int J Mol Sci. 23: PMID: 35563446
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  12. Differential RNA aptamer affinity profiling on plasma as a potential diagnostic tool for bladder cancer.  |  Fjelstrup, S., et al. 2022. NAR Cancer. 4: zcac025. PMID: 36004048
  13. A mutated glycosaminoglycan-binding domain functions as a novel probe to selectively target heparin-like epitopes on tumor cells.  |  Xu, Y., et al. 2022. J Biol Chem. 298: 102609. PMID: 36265583

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Biotin LC hydrazide, 100 mg

sc-257155
100 mg
$141.00

Do you have a protocol for using: Biotin LC hydrazide (CAS 109276-34-8)? Instructions for use?

Asked by: Frankvella
Thank you for your question. We have not used this product in-house, and do not have a recommended protocol for use.
Answered by: Technical Support
Date published: 2020-03-31
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Rated 5 out of 5 by from Cho E; et alCho E; et al. (PubMed ID: 24445053) utilized Biotin LC hydrazide to tag succinoglycans and monomers by reductive amination. -SCBT Publication Review
Date published: 2015-04-02
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Biotin LC hydrazide is rated 5.0 out of 5 by 1.
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