Bio-16-ddUTP . tetralithium salt
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Bio-16-ddUTP tetralithium salt aslo know as Biotin-16-2′,3′-dideoxyuridine-5′-triphosphate can be used for 3′-end labeling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3′-terminal addition of a single Bio-16-ddUMP. Biotin-labeled oligonucleotides produced by this reaction are suitable as hybridization probes. Biotin-labeled cDNA produced by this reaction can be used in nucleic acid array assays. Biotin-labeled DNA in hybridization assays can be detected by a reporter molecule linked to streptavidin, avidin, or an anti-biotin antibody. Such a complex can be detected directly for example, by the excitation of a fluorophore conjugated to streptavidin, or indirectly, such as by using an enzyme conjugate that can produce an insoluble colored precipitate. Bio-16-ddUTP tetralithium salt is also a substrate for Taq DNA Polymerase and other DNA polymerases.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bio-16-ddUTP . tetralithium salt, 25 nmol | sc-505887 | 25 nmol | $416.00 |