Bikunin Antibody (3F1): sc-517024

Bikunin Antibody (3F1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Bikunin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Bikunin antibody (3F1) is available as the non-conjugated format. The AMBP (α-1-Microglobulin/bikunin precursor) gene encodes a protein precursor that is cleaved to produce two distinct proteins, α-1-Microglobulin and Bikunin. Bikunin, also known as inter-alpha-trypsin inhibitor light chain or urinary trypsin inhibitor, plays a crucial role in regulating proteolytic activity in the extracellular matrix. Bikunin consists of a light inhibitory chain covalently linked to two heavy chains, forming the inter-alpha-inhibitor complex, which exists in high concentrations in human plasma. This complex stabilizes the extracellular matrix and prevents excessive proteolysis, which can lead to tissue damage and inflammation. Bikunin is expressed in various human tissues, including kidney, heart, liver, lung, and pancreas, and resides in granules of connective tissue mast cells. Bikunin appears on the surface of pancreatic acinar cells, where Bikunin may help prevent autodigestion by exocrine enzymes such as trypsinogen and chymotrypsinogen. Additionally, Bikunin functions as a potent inhibitor of calcium oxalate crystallization, suggesting involvement in the pathophysiology of renal diseases, including urolithiasis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Bikunin Antibody (3F1) References:

1. Role of inter-alpha-inhibitor and its related proteins in experimentally induced calcium oxalate urolithiasis. Localization of proteins and expression of bikunin gene in the rat kidney.  |  Atmani, F., et al. 1999. Urol Res. 27: 63-7. PMID: 10092155
2. Immunohistochemical demonstration of inter-alpha-trypsin inhibitor light chain (bikunin) in human mast cells.  |  Ide, H., et al. 1999. Cell Tissue Res. 297: 149-54. PMID: 10398892
3. Inflammation-induced systemic proteolysis of inter-alpha-inhibitor in plasma from patients with sepsis.  |  Balduyck, M., et al. 2000. J Lab Clin Med. 135: 188-98. PMID: 10695665
4. Expression of inter-alpha-trypsin inhibitor light chain (bikunin) in human pancreas.  |  Itoh, H., et al. 1996. J Biochem. 120: 271-5. PMID: 8889810
5. Posttranslational modifications of human inter-alpha-inhibitor: identification of glycans and disulfide bridges in heavy chains 1 and 2.  |  Olsen, EH., et al. 1998. Biochemistry. 37: 408-16. PMID: 9425062
6. Inter-alpha-inhibitor in calcium stones.  |  Dawson, CJ., et al. 1998. Clin Sci (Lond). 95: 187-93. PMID: 9680501

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bikunin Antibody (3F1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Bikunin Antibody (3F1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bikunin Antibody (3F1) Specifications

Alternate Product Name: Bikunin

Product Clone Name: 3F1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 19-352

Epitope Target: Bikunin

Epitope Species: human

Product Reactivity/Detection: Bikunin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Bikunin Antibody (3F1): N/A