Date published: 2025-12-10

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BID Antibody (40): sc-135847

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Datasheets
  • BID Antibody (40) is a mouse monoclonal IgG1, cited in 6 publications, provided at 50 µg/500 µl
  • raised against amino acids 46-168 of BID of mouse origin
  • recommended for detection of BID of mouse origin by WB and IP
  • See BID (F-5): sc-515616 for BID antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for BID Antibody (40). This work is in progress.

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BID Antibody (40) is a mouse monoclonal IgG1 antibody that detects BID in mouse samples through western blotting (WB) and immunoprecipitation (IP) applications. BID, a crucial member of the Bcl-2 family, plays a significant role in the regulation of apoptosis, acting as a pro-apoptotic factor that can promote cell death in response to various cellular signals. BID′s importance lies in its ability to dimerize with anti-apoptotic proteins like Bcl-2 and Bcl-x, effectively counteracting their protective effects and facilitating the apoptotic process. The presence of a BH3 domain in BID is essential for this dimerization, allowing BID to interact with other proteins in the apoptotic pathway. BID is predominantly localized in the cytosol but can also be found in membrane fractions, highlighting BID′s dynamic role in cellular signaling. BID is highly expressed in the kidney and is also detectable in other tissues such as the brain, spleen, liver, testis, and lung, indicating BID′s widespread influence on cell survival and death across various biological contexts. Anti-BID antibody (40) is an invaluable tool for researchers studying apoptosis and its implications in health and disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

BID Antibody (40)

sc-135847
50 µg/500 µl
$316.00

I am using BID (40): sc-135847 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Germaine
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from Robust band seen in WB using mouse macrophagesRobust band seen in WB using mouse macrophages stimulated with LPS/IFN- . -SCBT QC
Date published: 2014-07-14
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BID Antibody (40) is rated 3.0 out of 5 by 1.
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