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Bicinchoninic acid disodium salt (CAS 979-88-4)

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Alternate Names:
BCA; Bicinchonic acid, disodium salt
Application:
Bicinchoninic acid disodium salt is a photometric reagent for protein assay
CAS Number:
979-88-4
Purity:
≥98%
Molecular Weight:
388.29
Molecular Formula:
C20H10Na2N2O4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Bicinchoninic acid disodium salt (BCA) is a colorimetric reagent used for determination of reducing sugar and protein though Copper(I). Bicinchoninic acid disodium salt and Copper(I) form a stable 2:1 complex that has an absorbance maximum at 562 nm. It is used as a reagent in the colorimetric BCA protein quantitation assay in which Copper(II) is converted to Copper(I) as a function of protein concentration under alkaline conditions.


Bicinchoninic acid disodium salt (CAS 979-88-4) References

  1. Influence of wettability and surface activity on release behavior of hydrophilic substances from lipid matrices.  |  Koennings, S., et al. 2007. J Control Release. 119: 173-81. PMID: 17412444
  2. Towards controlled release of BDNF--manufacturing strategies for protein-loaded lipid implants and biocompatibility evaluation in the brain.  |  Koennings, S., et al. 2007. J Control Release. 119: 163-72. PMID: 17428570
  3. Determination of copper(I) and copper(II) ions after complexation with bicinchoninic acid by CE.  |  Basheer, C. and Lee, HK. 2007. Electrophoresis. 28: 3520-5. PMID: 17828801
  4. The mechanism of protein release from triglyceride microspheres.  |  Zaky, A., et al. 2010. J Control Release. 147: 202-10. PMID: 20659511
  5. Insights on antioxidant assays for biological samples based on the reduction of copper complexes-the importance of analytical conditions.  |  Marques, SS., et al. 2014. Int J Mol Sci. 15: 11387-402. PMID: 24968275
  6. Stabilization of Cu(I) for binding and calorimetric measurements in aqueous solution.  |  Johnson, DK., et al. 2015. Dalton Trans. 44: 16494-505. PMID: 26327397
  7. Sodium polyanethol sulfonate (SPS) falsifies protein staining and quantification and how to solve this problem.  |  Prax, M., et al. 2015. J Microbiol Methods. 118: 176-81. PMID: 26456688
  8. Rapid determination of trace copper in animal feed based on micro-plate colorimetric reaction and statistical partitioning correction.  |  Niu, Y., et al. 2017. Food Chem. 221: 1406-1414. PMID: 27979108
  9. A Low-Volume, Parallel Copper-Bicinchoninic Acid (BCA) Assay for Glycoside Hydrolases.  |  Arnal, G., et al. 2017. Methods Mol Biol. 1588: 3-14. PMID: 28417356
  10. Simultaneous quantification of total carbohydrate and protein amounts from aqueous solutions by the sulfuric acid ultraviolet absorption method (SA-UV method).  |  Ehnert, S., et al. 2021. Anal Chim Acta. 1174: 338712. PMID: 34247739
  11. Determination of N-Acetyl-l-cysteine Ethyl Ester (NACET) by Flow Injection Analysis and Spectrophotometric Detection Using Different Thiol-Sensitive Ligands.  |  Kukoc-Modun, L., et al. 2021. Molecules. 26: PMID: 34833918
  12. Dataset on the effects of environmentally relevant humic acid concentrations on the liver protein profile in Japanese medaka (Oryzias latipes).  |  Yurchenko, VV., et al. 2022. Data Brief. 40: 107796. PMID: 35036493
  13. BioFiber: An advanced fibrous textured dressing to manage exudate in severe wounds.  |  Maria Tottoli, E., et al. 2022. Int J Pharm. 625: 122073. PMID: 35931393
  14. Mechanism and Efficacy of Cu2O-Treated Fabric.  |  Benmamoun, Z., et al. 2022. Antibiotics (Basel). 11: PMID: 36421277
  15. A quantitative test for copper using bicinchoninic acid.  |  Brenner, AJ. and Harris, ED. 1995. Anal Biochem. 226: 80-4. PMID: 7785783

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Bicinchoninic acid disodium salt, 1 g

sc-278767
1 g
$102.00

Bicinchoninic acid disodium salt, 50 g

sc-278767A
50 g
$265.00

Bicinchoninic acid disodium salt, 250 g

sc-278767C
250 g
$979.00

Bicinchoninic acid disodium salt, 500 g

sc-278767B
500 g
$2968.00