βB2-crystallin Antibody (B-12): sc-376006

βB2-crystallin Antibody (B-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects βB2-crystallin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-βB2-crystallin antibody (B-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. βB2-crystallin plays a crucial role in maintaining the structural integrity and transparency of the eye lens, which is essential for proper vision. Located primarily in the lens fibers, βB2-crystallin contributes to the refractive properties of the lens by forming a highly organized structure that minimizes light scattering. This organization is vital for the lens′s ability to focus light accurately onto the retina, thereby ensuring clear vision. βB2-crystallin′s ability to form dimers and higher-order aggregates is critical for its function, as these interactions help stabilize the lens′s refractive index and prevent cataract formation. Additionally, βB2-crystallin is part of a larger family of crystallins, which includes several other beta and gamma crystallins, all of which work together to maintain lens transparency and refractive properties.

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βB2-crystallin Antibody (B-12) References:

1. The short 5' untranslated region of the betaA3/A1-crystallin mRNA is responsible for leaky ribosomal scanning.  |  Werten, PJ., et al. 1999. Mol Biol Rep. 26: 201-5. PMID: 10532316
2. Structure of the crystallins.  |  Slingsby, C. and Clout, NJ. 1999. Eye (Lond). 13 (Pt 3b): 395-402. PMID: 10627816
3. Alpha-crystallin.  |  Horwitz, J. 2003. Exp Eye Res. 76: 145-53. PMID: 12565801
4. Desminopathies in muscle disease.  |  Paulin, D., et al. 2004. J Pathol. 204: 418-27. PMID: 15495235
5. Transparency and non-refractive functions of crystallins--a proposal.  |  Bhat, SP. 2004. Exp Eye Res. 79: 809-16. PMID: 15642317
6. Beta-crystallin association.  |  Hejtmancik, JF., et al. 2004. Exp Eye Res. 79: 377-83. PMID: 15669142
7. Effect of oxidized betaB3-crystallin peptide on lens betaL-crystallin: interaction with betaB2-crystallin.  |  Udupa, EG. and Sharma, KK. 2005. Invest Ophthalmol Vis Sci. 46: 2514-21. PMID: 15980243
8. Peptides for targeting βB2-crystallin fibrils.  |  Ghaffari Sharaf, M., et al. 2017. Exp Eye Res. 165: 109-117. PMID: 28986145
9. Conformational stability of the deamidated and mutated human βB2-crystallin.  |  Velasco-Bolom, JL. and Dominguez, L. 2023. Biophys Chem. 296: 106986. PMID: 36905841
10. ATCUN-like Copper Site in βB2-Crystallin Plays a Protective Role in Cataract-Associated Aggregation.  |  Tovar-Ramírez, ME., et al. 2023. Inorg Chem. 62: 10592-10604. PMID: 37379524
11. Beta A3/A1-crystallin association: role of the N-terminal arm.  |  Hope, JN., et al. 1994. Protein Eng. 7: 445-51. PMID: 8177894
12. Association properties of betaB2- and betaA3-crystallin: ability to form dimers.  |  Hejtmancik, JF., et al. 1997. Protein Eng. 10: 1347-52. PMID: 9514125

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-βB2-crystallin Antibody (B-12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-βB2-crystallin Antibody (B-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

βB2-crystallin Antibody (B-12) Specifications

Alternate Product Name: βB2-crystallin

Product Clone Name: B-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-29

Epitope Target: βB2-crystallin

Epitope Species: human

Product Reactivity/Detection: βB2-crystallin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for βB2-crystallin Antibody (B-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred βB1-crystallin antibody is βB1-crystallin Antibody (H-3): sc-48335.