Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
beta Synuclein Double Nickase Plasmid (m): sc-430508-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- beta Synuclein Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- beta Synuclein Double Nickase Plasmid (m) and beta Synuclein Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Sncb. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: beta Synuclein Antibody (8): sc-136452
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
beta Synuclein Double Nickase Plasmid (m) | sc-430508-NIC | 20 µg | $410.00 | |||
beta Synuclein Double Nickase Plasmid (m2) | sc-430508-NIC-2 | 20 µg | $410.00 |
Sncb encodes beta-synuclein, a presynaptic neuronal protein that associates with synaptic vesicles and modulates neurotransmitter release and synaptic plasticity. In mouse brain, beta-synuclein participates in pathways governing vesicle trafficking, membrane curvature, and proteostasis, and can influence the aggregation behavior of other synuclein family members. Altered synuclein homeostasis has been linked to neurodegenerative mechanisms involving protein misfolding, axonal transport defects, and synaptic dysfunction. Sncb is therefore widely used as a molecular handle to dissect synapse maintenance, neuronal stress responses, and synuclein network interactions in vivo and in cultured neurons.
beta Synuclein Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Sncb locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Sncb. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Sncb function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Sncb-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.