β-Nicotinamide adenine dinucleotide-d4 (d3 major) (CAS 53-84-9 (unlabeled))

β-Nicotinamide adenine dinucleotide-d4 (d3 major) is a labeled analog of β-Nicotinamide adenine dinucleotide (NAD+), where deuterium atoms replace some of the hydrogen atoms, predominantly present as the d3 isotopologue. This modification enhances its stability and makes it useful for tracing and studying metabolic processes in biochemistry and molecular biology research. The incorporation of deuterium allows for the detailed analysis of enzymatic reactions involving NAD+, particularly those linked to energy transfer and redox reactions that are fundamental to cellular metabolism. Researchers utilize this isotopically labeled compound to explore the dynamics of NAD+ in various pathways, enabling precise measurement of reaction kinetics and understanding the role of NAD+ in regulatory mechanisms. By tracing the movement and conversion of NAD+ within cells, it helps in elucidating complex biochemical pathways and contributes to a deeper understanding of cellular metabolism.