Anti-beta-glucosidase Antibody (B-6) recommended for detection of β-glucosidase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-β-glucosidase Antibody (B-6): sc-166407

Anti-β-glucosidase Antibody (B-6) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-β-glucosidase Antibody (B-6) is a mouse monoclonal IgG2b κ β-glucosidase antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 237-536 mapping at the C-terminus of β-glucosidase of human origin
    • Anti-beta-glucosidase Antibody (B-6) is recommended for detection of β-glucosidase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-beta-glucosidase Antibody (B-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-glucosidase (B-6): sc-166407.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for β-glucosidase Antibody (B-6) for WB and IHC(P) applications. These reagents are now offered in bundles with β-glucosidase Antibody (B-6) (see ordering information below).
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I want to use β-glucosidase Antibody (B-6; (sc-166407) for Western Blot. What dilution to use?

Asked by: CCOB
Thank you for your question. We recommend a starting dilution 1:100, the dilution range spans from 1:100 to 1:1000 hand has to be determined.
Answered by: Tech Support Europe
Date published: 2021-07-23

I am using β-glucosidase (B-6): sc-166407 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great IF staining results We used this antibody for Immunoflourescence imaging and were able to take nice pictures from the resulting signal
Date published: 2017-02-03
Rated 5 out of 5 by from Robust signal in WB in mouse liver and rat Robust signal in WB in mouse liver and rat liver tissue extracts. -SCBT QC
Date published: 2015-07-12
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic staining Excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human heart muscle tissue. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from Strong band in WB in human transfected 293T Strong band in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-31
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