Anti-β-glucosidase Antibody (B-6): sc-166407
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- Anti-β-glucosidase Antibody (B-6) is a mouse monoclonal IgG2b κ β-glucosidase antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 237-536 mapping at the C-terminus of β-glucosidase of human origin
- Anti-beta-glucosidase Antibody (B-6) is recommended for detection of β-glucosidase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-beta-glucosidase Antibody (B-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-glucosidase (B-6): sc-166407.
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for β-glucosidase Antibody (B-6) for WB and IHC(P) applications. These reagents are now offered in bundles with β-glucosidase Antibody (B-6) (see ordering information below).
QUICK LINKS
SEE ALSO...
- Additional β-glucosidase Antibodies including β-glucosidase 2
- ChemCruz™ Chemical beta-glucosidase Inhibitors or beta-glucosidase Substrates for functional analysis of cellular responses to β-glucosidase
- Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional
I want to use β-glucosidase Antibody (B-6; (sc-166407) for Western Blot. What dilution to use?
Thank you for your question. We recommend a starting dilution 1:100, the dilution range spans from 1:100 to 1:1000 hand has to be determined.
Date published: 2021-07-23
I am using β-glucosidase (B-6): sc-166407 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here:
https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Date published: 2017-02-24
Rated 5 out of
5
by
Randy McDonald from
Great IF staining results
We used this antibody for Immunoflourescence imaging and were able to take nice pictures from the resulting signal
Date published: 2017-02-03
Rated 5 out of
5
by
HC from
Robust signal in WB in mouse liver and rat
Robust signal in WB in mouse liver and rat liver tissue extracts. -SCBT QC
Date published: 2015-07-12
Rated 5 out of
5
by
MS from
Excellent immunoperoxidase cytoplasmic staining
Excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human heart muscle tissue. -SCBT QC
Date published: 2015-04-03
Rated 5 out of
5
by
JR from
Strong band in WB in human transfected 293T
Strong band in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-31