Anti-beta-defensin 2 Antibody (2-RY8) recommended for detection of β-defensin 2 of human origin by WB, IP and ELISA

Anti-β-defensin 2 Antibody (2-RY8): sc-134314

Anti-β-defensin 2 Antibody (2-RY8) is rated 3.5 out of 5 by 4.
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Datasheets

    • Anti-β-defensin 2 Antibody (2-RY8) is a mouse monoclonal IgG1 (kappa light chain) β-defensin 2 antibody provided at 100 µg/ml
    • raised against a partial recombinant fragment of β-defensin 2 of human origin
    • recommended for detection of β-defensin 2 of human origin by WB, IP and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-defensin 2 (2-RY8): sc-134314.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for β-defensin 2 Antibody (2-RY8). This work is in progress.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with β-defensin 2 (2-RY8): sc-134314 antibody?

Asked by: AbPolly
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 1 out of 5 by from IHC Assay Review for Beta-Defensin-2 mAB Application: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: Specific stain not detected even at dilution factor of 10.
Date published: 2017-08-31
Rated 5 out of 5 by from western blot detection It worked with succes for western blot detect β-defensin 2,WB 1:1000,The samples were 293T cells,the band at 5kda.Recommend use 15% SDS-PAGE,since the MW is low.
Date published: 2017-08-12
Rated 5 out of 5 by from Western blot Western blot detection of good human cells, WB diluted 1:1000 clear band.
Date published: 2017-05-24
Rated 3 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2013-04-19
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