BES sodium salt (CAS 66992-27-6)

BES sodium salt, derived from BES (N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid), is a zwitterionic buffering agent widely used in biochemical and biophysical research for its ability to maintain stable pH levels, particularly effective within the range of pH 6.4 to 7.8. Its buffering capability stems from its action as both a proton donor and acceptor, which helps stabilize pH in reaction mixtures, crucial for maintaining the activity and structural integrity of enzymes and other sensitive biomolecules. This feature makes BES sodium salt particularly useful in enzyme kinetics and other biochemical assays where precise pH control is essential. Additionally, its non-interfering nature with the biological activity of proteins makes it an excellent choice for protein solubility studies and electrophoresis. BES sodium salt is also favored in cell culture applications, contributing to the optimal extracellular environment for cell growth by effectively managing the pH without disturbing the ionic balance of the medium. Moreover, its application extends to protein folding studies and ion exchange processes, benefiting from its effective buffering range and low UV absorbance, which is critical for experiments involving spectrophotometric analysis, ensuring minimal optical interference and enhanced result accuracy. This versatility underscores the importance of BES sodium salt as a fundamental component in various scientific research settings.