Anti-Bcl-7b Antibody (NQ-B25) recommended for detection of Bcl-7b of human origin by WB, IP and ELISA

Anti-Bcl-7b Antibody (NQ-B25): sc-134278

Anti-Bcl-7b Antibody (NQ-B25) is rated 3.5 out of 5 by 2.
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Datasheets

    • Anti-Bcl-7b Antibody (NQ-B25) is a mouse monoclonal IgG1 (kappa light chain) Bcl-7b antibody provided at 100 µg/ml
    • raised against recombinant Bcl-7b protein of human origin
    • recommended for detection of Bcl-7b of human origin by WB, IP and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Bcl-7b (NQ-B25): sc-134278.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Bcl-7b Antibody (NQ-B25). This work is in progress.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with Bcl-7b (NQ-B25): sc-134278 antibody?

Asked by: Trav11
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from Good antibody for Western Gave strong signal on Western blot but did have to reprobe blot a second night at a higher dilution of antibody. Not the greatest workflow but did get good, crisp bands. Maybe need to start with higher dilution first night.
Date published: 2017-10-04
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