Bcl-3 Antibody (150-3.5): sc-32741

Bcl-3 Antibody (150-3.5) is a hamster monoclonal IgG that detects Bcl-3, also known as B-cell lymphoma 3-encoded protein or BCL3, of mouse, rat, and human origin by western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). Raised against the full-length Bcl-3 of mouse origin, anti-Bcl-3 antibody (150-3.5) is non-cross-reactive with other Bcl isoforms. Bcl-3 (150-3.5) monoclonal antibody is available in both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Bcl-3 is a proto-oncogene that encodes a protein member of the IκB family, functioning as a transcriptional coactivator involved in regulating NF-κB-mediated gene expression. Bcl-3 interacts with p50 and p52 NF-κB homodimers, modulating immune responses, cell proliferation, and survival, which is crucial in understanding its role in oncogenesis. Bcl-3 is implicated in certain human B-cell leukemias and lymphomas, making Bcl-3 a significant target in cancer research. Also designated as B-cell leukemia/lymphoma 3, Bcl-3 dysregulation can contribute to abnormal cell growth. Studying Bcl-3 is essential for insights into NF-κB signaling pathways and the development of therapeutic strategies for related cancers.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Bcl-3 Antibody (150-3.5) References:

1. I kappa B gamma, a 70 kd protein identical to the C-terminal half of p110 NF-kappa B: a new member of the I kappa B family.  |  Inoue, J., et al. 1992. Cell. 68: 1109-20. PMID: 1339305
2. I kappa B interacts with the nuclear localization sequences of the subunits of NF-kappa B: a mechanism for cytoplasmic retention.  |  Beg, AA., et al. 1992. Genes Dev. 6: 1899-913. PMID: 1340770
3. Candidate proto-oncogene bcl-3 encodes a subunit-specific inhibitor of transcription factor NF-kappa B.  |  Wulczyn, FG., et al. 1992. Nature. 358: 597-9. PMID: 1501714
4. The NF-kappaB regulator Bcl-3 and the BH3-only proteins Bim and Puma control the death of activated T cells.  |  Bauer, A., et al. 2006. Proc Natl Acad Sci U S A. 103: 10979-84. PMID: 16832056
5. Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity.  |  Haskill, S., et al. 1991. Cell. 65: 1281-9. PMID: 1829648
6. Cloning of an NF-kappa B subunit which stimulates HIV transcription in synergy with p65.  |  Schmid, RM., et al. 1991. Nature. 352: 733-6. PMID: 1876189
7. Rel-associated pp40: an inhibitor of the rel family of transcription factors.  |  Davis, N., et al. 1991. Science. 253: 1268-71. PMID: 1891714
8. The rel-associated pp40 protein prevents DNA binding of Rel and NF-kappa B: relationship with I kappa B beta and regulation by phosphorylation.  |  Kerr, LD., et al. 1991. Genes Dev. 5: 1464-76. PMID: 1907941
9. Activation in vitro of NF-kappa B by phosphorylation of its inhibitor I kappa B.  |  Ghosh, S. and Baltimore, D. 1990. Nature. 344: 678-82. PMID: 2157987
10. Bcl-3 regulates UVB-induced apoptosis.  |  García, I., et al. 2013. Hum Cell. 26: 47-55. PMID: 23494744
11. B-cell-derived IL-10 promotes allergic sensitization in asthma regulated by Bcl-3.  |  Qian, G., et al. 2023. Cell Mol Immunol. 20: 1313-1327. PMID: 37653127
12. Bcl-3 regulates T cell function through energy metabolism.  |  Liu, H., et al. 2023. BMC Immunol. 24: 35. PMID: 37794349

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bcl-3 Antibody (150-3.5) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Bcl-3 Antibody (150-3.5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bcl-3 Antibody (150-3.5) Specifications

Alternate Product Name: BCL3

Product Clone Name: 150-3.5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG

Amino Acids:

Epitope Target: Bcl-3

Epitope Species: mouse

Product Reactivity/Detection: Bcl-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, ELISA

Vialing Information: Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Bcl-3 Antibody (150-3.5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin