Bcl-11a/b Antibody (F-10): sc-515783
- Bcl-11a/b Antibody (F-10) is a mouse monoclonal IgG1 provided at 200 µg/ml
- specific for an epitope mapping between amino acids 656-681 near the C-terminus of Bcl-11b of human origin
- Bcl-11a/b Antibody (F-10) is recommended for detection of Bcl-11a and Bcl-11b of human origin by WB, IP, IF and ELISA
- Anti-Bcl-11a/b Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for Bcl-11a/b Antibody (F-10) for WB applications. This reagent is now offered in a bundle with Bcl-11a/b Antibody (F-10) (see ordering information below).
Bcl-11a/b Antibody (F-10) is a mouse monoclonal IgG1 antibody that detects Bcl-11a and Bcl-11b proteins of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Bcl-11a/b antibody (F-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bcl-11a and Bcl-11b proteins are critical transcription factors involved in lymphopoiesis, which is the process of lymphocyte development, and play significant roles in the progression of hematopoietic malignancies. Bcl-11b is associated with T cell acute lymphoblastic leukemia, and disruption can lead to T cell lymphoma, highlighting the importance of these proteins in maintaining normal immune function. Bcl-11a is essential for postnatal development and normal lymphocyte formation, while Bcl-11a gene is frequently targeted by retroviral integration in myeloid leukemia, suggesting a potential role as a leukemia disease gene, particularly through interactions with other proteins such as Bcl-6. Bcl-11b interacts with metastasis-associated proteins MTA1 and MTA2, further emphasizing involvement in complex regulatory networks within the cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Bcl-11a/b Antibody (F-10) References:
- Direct Promoter Repression by BCL11A Controls the Fetal to Adult Hemoglobin Switch. | Liu, N., et al. 2018. Cell. 173: 430-442.e17. PMID: 29606353
- The transcription factor Bcl11b promotes both canonical and adaptive NK cell differentiation. | Holmes, TD., et al. 2021. Sci Immunol. 6: PMID: 33712472
- 14q32 rearrangements deregulating BCL11B mark a distinct subgroup of T-lymphoid and myeloid immature acute leukemia. | Di Giacomo, D., et al. 2021. Blood. 138: 773-784. PMID: 33876209
- Transcription factors Bcl11a and Bcl11b are required for the production and differentiation of cortical projection neurons. | Du, H., et al. 2022. Cereb Cortex. 32: 3611-3632. PMID: 34963132
- Single-cell deletion analyses show control of pro-T cell developmental speed and pathways by Tcf7, Spi1, Gata3, Bcl11a, Erg, and Bcl11b. | Zhou, W., et al. 2022. Sci Immunol. 7: eabm1920. PMID: 35594339
- HIC2 controls developmental hemoglobin switching by repressing BCL11A transcription. | Huang, P., et al. 2022. Nat Genet. 54: 1417-1426. PMID: 35941187
- BCL11B and the NuRD complex cooperatively guard T-cell fate and inhibit OPA1-mediated mitochondrial fusion in T cells. | Liao, R., et al. 2023. EMBO J. 42: e113448. PMID: 37737560
- ARID1B maintains mesenchymal stem cell quiescence via inhibition of BCL11B-mediated non-canonical Activin signaling. | Zhang, M., et al. 2024. Nat Commun. 15: 4614. PMID: 38816354
- BCL11A intellectual developmental disorder: defining the clinical spectrum and genotype-phenotype correlations. | Peron, A., et al. 2025. Eur J Hum Genet. 33: 312-324. PMID: 39448799
- A mutant BCL11B-N440K protein interferes with BCL11A function during T lymphocyte and neuronal development. | Okuyama, K., et al. 2024. Nat Immunol. 25: 2284-2296. PMID: 39487351
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bcl-11a/b Antibody (F-10) | sc-515783 | 200 µg/ml | $316.00 | |||
Bcl-11a/b Antibody (F-10): m-IgG1 BP-HRP Bundle | sc-531812 | 200 µg Ab; 20 µg BP | $354.00 | |||
Bcl-11a/b Antibody (F-10) AC | sc-515783 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Bcl-11a/b Antibody (F-10) HRP | sc-515783 HRP | 200 µg/ml | $316.00 | |||
Bcl-11a/b Antibody (F-10) FITC | sc-515783 FITC | 200 µg/ml | $330.00 | |||
Bcl-11a/b Antibody (F-10) PE | sc-515783 PE | 200 µg/ml | $343.00 | |||
Bcl-11a/b Antibody (F-10) Alexa Fluor® 488 | sc-515783 AF488 | 200 µg/ml | $357.00 | |||
Bcl-11a/b Antibody (F-10) Alexa Fluor® 546 | sc-515783 AF546 | 200 µg/ml | $357.00 | |||
Bcl-11a/b Antibody (F-10) Alexa Fluor® 594 | sc-515783 AF594 | 200 µg/ml | $357.00 | |||
Bcl-11a/b Antibody (F-10) Alexa Fluor® 647 | sc-515783 AF647 | 200 µg/ml | $357.00 | |||
Bcl-11a/b Antibody (F-10) Alexa Fluor® 680 | sc-515783 AF680 | 200 µg/ml | $357.00 | |||
Bcl-11a/b Antibody (F-10) Alexa Fluor® 790 | sc-515783 AF790 | 200 µg/ml | $357.00 |