BCIP/NBT Stock Solution, 50X
See product citations (39)
Application:
BCIP/NBT Stock Solution, 50X is a substrate for alkaline phosphatase (AP) based detection systems including western blotting
Molecular Weight:
NBT: MW = 817.7, BCIP toluidine salt: MW = 433.6
Molecular Formula:
NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP•C7H9N
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
QUICK LINKS
Ordering Information
Product Citations
Description
Technical Information
Safety Information
SDS & Certificate of Analysis
5-bromo, 4-chloro, 3-indolylphosphate (BCIP)/Nitro-Blue Tetrazolium (NBT) substrate, used for the localization of alkaline phosphatase (AP) labeled probes on western, northern, southern and dot blots. BCIP acts as the substrate for AP and reacts further after the dephosphorylation to give a dark-blue indigo-dye as an oxidation product; NBT serves herein as the oxidant and also gives a dark-blue dye, intensifying the color and making the detection more sensitive. Recommended for use with Crystal Mounting Medium (sc-24943).
BCIP/NBT Stock Solution, 50X References
- Hepatocyte growth factor system in the mouse uterus: variation across the estrous cycle and regulation by 17-beta-estradiol and progesterone. | Zhang, X. 2010. Biol Reprod. 82: 1037-48. PMID: 20147731
- Cellular localization of genes and proteins for tumor necrosis factor-α (TNF), TNF receptor types I and II in bovine endometrium. | Okuda, K., et al. 2010. Mol Cell Endocrinol. 330: 41-8. PMID: 20705117
- Oligodendrocyte PTEN is required for myelin and axonal integrity, not remyelination. | Harrington, EP., et al. 2010. Ann Neurol. 68: 703-16. PMID: 20853437
- Expression of engrailed-family genes in the jumping bristletail and discussion on the primitive pattern of insect segmentation. | Nakagaki, Y., et al. 2015. Dev Genes Evol. 225: 313-8. PMID: 26245911
- A Point-of-Need infrared mediated PCR platform with compatible lateral flow strip for HPV detection. | Liu, W., et al. 2017. Biosens Bioelectron. 96: 213-219. PMID: 28499198
- Immunolocalization of IAA Using an Anti-IAA-C-Antibody Raised Against Carboxyl-Linked IAA. | Nishimura, T. and Koshiba, T. 2019. Methods Mol Biol. 1924: 165-172. PMID: 30694474
- Developmental neurotoxicity of reserpine exposure in zebrafish larvae (Danio rerio). | Wang, S., et al. 2019. Comp Biochem Physiol C Toxicol Pharmacol. 223: 115-123. PMID: 31128281
- Growth and accelerated differentiation of mesenchymal stem cells on graphene oxide/poly-l-lysine composite films. | Qi, W., et al. 2014. J Mater Chem B. 2: 5461-5467. PMID: 32261766
- A new histochemical stain for neutrophilic leukocyte alkaline phosphatase. | Wolf, PL., et al. 1968. Enzymologia. 35: 154-6. PMID: 4176788
- A test for bacterial alkaline phosphatase: use in rapid identification of Serratia organisms. | Wolf, PL., et al. 1973. Clin Chem. 19: 1248-9. PMID: 4758599
- Substrates for cytochemical demonstration of enzyme activity. II. Some dihalo-3-indolyl phosphates and sulfates. | Horwitz, JP., et al. 1966. J Med Chem. 9: 447. PMID: 5960940
- Adsorbed BMP-2 in polyelectrolyte multilayer films for enhanced early osteogenic differentiation of mesenchymal stem cells | Cai, P., Xue, Z., Qi, W., & Wang, H. 2013. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 434: 110-117.
Substrate of:
HRP and AP.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BCIP/NBT Stock Solution, 50X, 8 ml | sc-24981 | 8 ml | $199.00 |