用法 :
Preparation of staining solution:
•For all applications except Digoxigenin (DIG) System, add 200 µl of BCIP/NBT stock solution to the following room temperature buffer: 10 ml 0.1 M Tris Hydrochloride, pH 9.5, 0.1 M Sodium Chloride, and 0.05 M Magnesium Chloride.
For DIG Systems specifically, do not include Magnesium Chloride in the detection buffer, this may cause spotty background on the membrane after the detection procedure. Add only 200 µl of the BCIP stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20°C), and 0.1 M NaCl
•Prepare solution immediately prior to use.
•Discard if solution is turbid or purple.