Date published: 2026-4-1

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BCIP/INT, 1X

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Application:
BCIP/INT, 1X is a ready to use substrate for alkaline phosphate (AP) based detection systems. Suitable for IHC and ICC applications
Molecular Weight:
INT: Mr = 505.7; BCIP toluidine salt: Mr = 433.6
Molecular Formula:
INT: C19H13ClN5O2; BCIP: C8H6NO4BrClP•C7H9N
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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BCIP/INT, 1X is a substrate for alkaline phosphate (AP) based detection systems. BCIP acts as the substrate for AP, INT enhances the yellow-brown final color of the precipitates at the reaction sites. BCIP/INT, 1X is suitable for immunohistochemistry, immunocytochemistry and in situ hybridization procedures in both manual and automated systems.


BCIP/INT, 1X References

  1. Morphogenesis of prechordal plate and notochord requires intact Eph/ephrin B signaling.  |  Chan, J., et al. 2001. Dev Biol. 234: 470-82. PMID: 11397014
  2. Alternative splicing as a novel of means of regulating the expression of therapeutic genes.  |  Hayes, GM., et al. 2002. Cancer Gene Ther. 9: 133-41. PMID: 11857030
  3. FGFR4 signaling is a necessary step in limb muscle differentiation.  |  Marics, I., et al. 2002. Development. 129: 4559-69. PMID: 12223412
  4. Multicolour whole-mount in situ hybridization to Drosophila embryos.  |  Hauptmann, G. and Gerster, T. 1996. Dev Genes Evol. 206: 292-5. PMID: 24173569
  5. Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos.  |  Hauptmann, G., et al. 2016. J Vis Exp. e53830. PMID: 26862978
  6. A smart fluorescence nanoprobe for the detection of cellular alkaline phosphatase activity and early osteogenic differentiation.  |  Cao, FY., et al. 2016. Nanomedicine. 12: 1313-22. PMID: 26961462
  7. Anatomic viral detection is automated: the application of a robotic molecular pathology system for the detection of DNA viruses in anatomic pathology substrates, using immunocytochemical and nucleic acid hybridization techniques.  |  Montone, KT., et al. 1989. Yale J Biol Med. 62: 141-58. PMID: 2773514
  8. Expression and function of microRNA-9 in the mid-hindbrain area of embryonic chick.  |  Alwin Prem Anand, A., et al. 2018. BMC Dev Biol. 18: 3. PMID: 29471810
  9. Substrates for cytochemical demonstration of enzyme activity. II. Some dihalo-3-indolyl phosphates and sulfates.  |  Horwitz, JP., et al. 1966. J Med Chem. 9: 447. PMID: 5960940

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

BCIP/INT, 1X, 100 ml

sc-24980
100 ml
$80.00