BCAR3 Antibody (3G4): sc-293346
- BCAR3 Antibody (3G4) is a mouse monoclonal IgG2a κ BCAR3 antibody provided at 100 µg/ml
- raised against amino acids 266-373 of BCAR3 of human origin
- recommended for detection of BCAR3 of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for BCAR3 Antibody (3G4). This work is in progress.
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BCAR3 Antibody (3G4) is a mouse monoclonal IgG2a kappa light chain antibody that detects BCAR3 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-BCAR3 antibody (3G4) is available as the non-conjugated format. BCAR3, also known as breast cancer anti-estrogen resistance 3, plays a crucial role in the development of antiestrogen resistance in breast cancer, particularly in ZR-75-1 cell lines. BCAR3 is characterized by an 825-amino acid structure, which includes a Src homology 2 (SH2) domain, allowing BCAR3 to interact with various signaling pathways. BCAR3 is involved in regulating R-Ras, which in turn modulates the levels of insulin receptor substrate 1 (IRS-1) in breast cancer cells, highlighting BCAR3′s importance in cellular signaling and cancer progression. BCAR3 expression is observed in multiple human tissues, including the heart, placenta, skeletal muscle, spleen, prostate, testis, ovary, small intestine, colon, and fetal kidney, as well as in tamoxifen-resistant cancer cell lines. BCAR3 interacts with p130Cas, enhancing Src activation and promoting cell migration, which is critical for understanding the mechanisms of cancer metastasis and resistance to therapy.
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BCAR3 Antibody (3G4) References:
- p130Cas regulates the activity of AND-34, a novel Ral, Rap1, and R-Ras guanine nucleotide exchange factor. | Gotoh, T., et al. 2000. J Biol Chem. 275: 30118-23. PMID: 10896938
- Synergistic promotion of c-Src activation and cell migration by Cas and AND-34/BCAR3. | Riggins, RB., et al. 2003. J Biol Chem. 278: 28264-73. PMID: 12740391
- AND-34/BCAR3, a GDP exchange factor whose overexpression confers antiestrogen resistance, activates Rac, PAK1, and the cyclin D1 promoter. | Cai, D., et al. 2003. Cancer Res. 63: 6802-8. PMID: 14583477
- Breast cancer oestrogen independence mediated by BCAR1 or BCAR3 genes is transmitted through mechanisms distinct from the oestrogen receptor signalling pathway or the epidermal growth factor receptor signalling pathway. | Dorssers, LC., et al. 2005. Breast Cancer Res. 7: R82-92. PMID: 15642172
- The R-Ras GTPase mediates cross talk between estrogen and insulin signaling in breast cancer cells. | Yu, Y., et al. 2006. Mol Cell Biol. 26: 6372-80. PMID: 16914723
- Identification of BCAR3 by a random search for genes involved in antiestrogen resistance of human breast cancer cells. | van Agthoven, T., et al. 1998. EMBO J. 17: 2799-808. PMID: 9582273
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BCAR3 Antibody (3G4) | sc-293346 | 100 µg/ml | $322.00 |