BBS10 Double Nickase Plasmid (h): sc-408538-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BBS10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BBS10 Double Nickase Plasmid (h) and BBS10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BBS10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BBS10 Antibody (A-3): sc-518239
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BBS10 Double Nickase Plasmid (h) | sc-408538-NIC | 20 µg | $410.00 | |||
BBS10 Double Nickase Plasmid (h2) | sc-408538-NIC-2 | 20 µg | $410.00 |
BBS10 encodes a chaperonin-like protein that functions within the BBSome/chaperonin complex to support ciliogenesis and the assembly/trafficking of ciliary membrane proteins. Through its role in primary cilium formation and intraflagellar transport–related processes, BBS10 influences cilia-dependent signaling networks, including Hedgehog and other sensory pathways that coordinate cell polarity, proliferation, and differentiation. Disruption of BBS10 impairs ciliary structure and receptor trafficking, linking it to pleiotropic phenotypes characteristic of ciliopathies. Human BBS10 is therefore widely studied in models of Bardet–Biedl syndrome and related cilia-associated disorders to interrogate mechanisms of organ development, metabolism, and sensory function.
BBS10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BBS10 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BBS10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BBS10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BBS10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.