basonuclin Antibody (1F4): sc-517114

basonuclin Antibody (1F4) is a mouse monoclonal IgG2b kappa light chain antibody that detects basonuclin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-basonuclin antibody (1F4) is available as a non-conjugated format. basonuclin protein, a zinc finger transcription factor, plays a crucial role in regulating ribosomal DNA transcription, which is essential for ribosome biogenesis and protein synthesis in cells. basonuclin expression is highly restricted to specific tissues, being predominantly found in keratinocytes of the basal layer of the epidermis, the outer sheath of hair follicles, and in germ cells of the testis and ovary. basonuclin is associated with chromatin throughout the cell cycle, including during mitosis, but is absent in post-mitotic cells, indicating importance in cell proliferation and differentiation. In the epidermis, basonuclin is primarily localized in the cytoplasm but translocates to the nuclei of basal cells during various stages of keratinocyte growth, suggesting a role in regulating gene expression during cellular development. By enhancing rRNA synthesis through interaction with rDNA promoters and modulating RNA polymerase I activity via zinc finger domain, basonuclin may serve as a cell-type-specific transcription factor, making basonuclin a significant player in cellular growth and function.

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basonuclin Antibody (1F4) References:

1. Basonuclin, a zinc finger protein of keratinocytes and reproductive germ cells, binds to the rRNA gene promoter.  |  Iuchi, S. and Green, H. 1999. Proc Natl Acad Sci U S A. 96: 9628-32. PMID: 10449744
2. Basonuclin is associated with the ribosomal RNA genes on human keratinocyte mitotic chromosomes.  |  Tseng, H., et al. 1999. J Cell Sci. 112 Pt 18: 3039-47. PMID: 10462520
3. Basonuclin in murine corneal and lens epithelia correlates with cellular maturation and proliferative ability.  |  Tseng, H., et al. 1999. Differentiation. 65: 221-7. PMID: 10653358
4. Alternative subcellular locations of keratinocyte basonuclin.  |  Iuchi, S., et al. 2000. Exp Dermatol. 9: 178-84. PMID: 10839715
5. Function of basonuclin in increasing transcription of the ribosomal RNA genes during mouse oogenesis.  |  Tian, Q., et al. 2001. Development. 128: 407-16. PMID: 11152639
6. Basonuclin: a novel mammalian maternal-effect gene.  |  Ma, J., et al. 2006. Development. 133: 2053-62. PMID: 16624857
7. Search for basonuclin target genes.  |  Wang, J., et al. 2006. Biochem Biophys Res Commun. 348: 1261-71. PMID: 16919236
8. Translocation of the zinc finger protein basonuclin from the mouse germ cell nucleus to the midpiece of the spermatozoon during spermiogenesis.  |  Mahoney, MG., et al. 1998. Biol Reprod. 59: 388-94. PMID: 9687312
9. Basonuclin, a zinc finger protein associated with epithelial expansion and proliferation.  |  Tseng, H. 1998. Front Biosci. 3: D985-8. PMID: 9727087

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-basonuclin Antibody (1F4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-basonuclin Antibody (1F4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

basonuclin Antibody (1F4) Specifications

Alternate Product Name: basonuclin

Product Clone Name: 1F4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 599-705

Epitope Target: basonuclin

Epitope Species: human

Product Reactivity/Detection: basonuclin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for basonuclin Antibody (1F4): N/A