BAP31 CRISPR Activation Plasmid (h): sc-402421-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BAP31 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- BAP31 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by BAP31 CRISPR Activation Plasmid (h) and BAP31 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the BCAP31 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BAP31 Antibody (D-6): sc-393810
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BAP31 CRISPR Activation Plasmid (h) | sc-402421-ACT | 20 µg | $397.00 | |||
BAP31 CRISPR Activation Plasmid (h2) | sc-402421-ACT-2 | 20 µg | $397.00 |
BCAP31 encodes the human BAP31 protein, a multi-pass endoplasmic reticulum (ER) membrane chaperone that regulates the trafficking and quality control of newly synthesized membrane proteins. BAP31 participates in ER-to-Golgi transport and ER-associated degradation (ERAD), linking secretory pathway flux with unfolded protein response signaling and proteostasis maintenance. It also interfaces with apoptosis and calcium homeostasis at ER–mitochondria contact sites, influencing cellular stress sensitivity. Dysregulated BCAP31/BAP31 function has been implicated in altered protein handling and cell survival programs relevant to neurodevelopmental and oncogenic contexts.
BAP31 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous BCAP31 expression without altering the underlying DNA sequence.
BAP31 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the BCAP31 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the BCAP31 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous BAP31 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native BCAP31 locus and enabling the study of BAP31-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of BAP31 pathway restoration in tumor cells with silenced or reduced BCAP31 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.