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Bafilomycin A1 (CAS 88899-55-2)

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Alternate Names:
Bafilomycin A1 is also known as (3Z,5E,7R,8S,9S,11E,13E,15S,16R)-8-Hydroxy-16-[(1S,2R,3S)-2-hydroxy-1-methyl-3-[(2R,4R,5S,6R)-tetrahydro-2,4-dihydroxy-5-methyl-6-(1-methylethyl)-2H-pyran-2-yl]butyl]-3,15-dimethoxy-5,7,9,11-tetramethyloxacyclohexadeca-3,5,11,13-tetraen-2-one
Application:
Bafilomycin A1® is a macrolide antibiotic and potent and selective inhibitor of vacuolar-type H+ ATPase (V-ATPase)
CAS Number:
88899-55-2
Purity:
≥95%
Molecular Weight:
622.83
Molecular Formula:
C35H58O9
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Bafilomycin A1 is a potent and selective inhibitor of V-ATPase (vacuolar-type H+ ATPase) and belongs to a class of membrane ATPase inhibitors. Bafilomycin A1 can inhibit E1E2 ATPases from sarcoplasmic reticulum, Escherichia coli, and ox brain versus F1F0 ATPases found in bacteria and mitochondria. Some of the more bafilomycin A1 affected ATPases include Neurospora and plant vacuoles, as well as chromaffin granules. Additionally, Bafilomycin A1 prevents lysosomal cholesterol trafficking in macrophages and can be used to distinguish different types of ATPases. Research shows that Bafilomycin A1 can inhibit macroautophagy and promote apoptosis in colon cancer cells.


Bafilomycin A1 (CAS 88899-55-2) References

  1. Inhibition of macroautophagy by bafilomycin A1 lowers proliferation and induces apoptosis in colon cancer cells.  |  Wu, YC., et al. 2009. Biochem Biophys Res Commun. 382: 451-6. PMID: 19289106
  2. Bafilomycin A1 targets both autophagy and apoptosis pathways in pediatric B-cell acute lymphoblastic leukemia.  |  Yuan, N., et al. 2015. Haematologica. 100: 345-56. PMID: 25512644
  3. Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells.  |  Bowman, EJ., et al. 1988. Proc Natl Acad Sci U S A. 85: 7972-6. PMID: 2973058
  4. Chloroquine inhibits autophagic flux by decreasing autophagosome-lysosome fusion.  |  Mauthe, M., et al. 2018. Autophagy. 14: 1435-1455. PMID: 29940786
  5. Bafilomycin A1 alleviates depression‑like symptoms in chronic unpredictable mild stress rats.  |  Wang, Z., et al. 2018. Mol Med Rep. 18: 4587-4594. PMID: 30221667
  6. The release and activity of HMGB1 in ferroptosis.  |  Wen, Q., et al. 2019. Biochem Biophys Res Commun. 510: 278-283. PMID: 30686534
  7. Bafilomycin-A1 and ML9 Exert Different Lysosomal Actions to Induce Cell Death.  |  Shaikh, S., et al. 2019. Curr Mol Pharmacol. 12: 261-271. PMID: 30854984
  8. Bafilomycin A1 targets patient-derived CD34+CD19+ leukemia stem cells.  |  Xu, L., et al. 2020. Haematologica. 105: e17-e21. PMID: 31097633
  9. Bafilomycin A1 and U18666A Efficiently Impair ZIKV Infection.  |  Sabino, C., et al. 2019. Viruses. 11: PMID: 31174294
  10. Bafilomycin A1 Accelerates Chronic Refractory Wound Healing in db/db Mice.  |  Wang, F., et al. 2020. Biomed Res Int. 2020: 6265701. PMID: 32714982
  11. Molecular basis of V-ATPase inhibition by bafilomycin A1.  |  Wang, R., et al. 2021. Nat Commun. 12: 1782. PMID: 33741963
  12. SARS-CoV-2 Omicron variant shows less efficient replication and fusion activity when compared with Delta variant in TMPRSS2-expressed cells.  |  Zhao, H., et al. 2022. Emerg Microbes Infect. 11: 277-283. PMID: 34951565
  13. [Audiovisual recordings of electromyographic examinations (author's transl)].  |  Hacke, W. 1978. EEG EMG Z Elektroenzephalogr Elektromyogr Verwandte Geb. 9: 179-81. PMID: 81128
  14. Bafilomycin A1, a specific inhibitor of vacuolar-type H(+)-ATPase, blocks lysosomal cholesterol trafficking in macrophages.  |  Furuchi, T., et al. 1993. J Biol Chem. 268: 27345-8. PMID: 8262974

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Bafilomycin A1, 100 µg

sc-201550
100 µg
$96.00

Bafilomycin A1, 1 mg

sc-201550A
1 mg
$250.00

Bafilomycin A1, 5 mg

sc-201550B
5 mg
$750.00

Bafilomycin A1, 10 mg

sc-201550C
10 mg
$1428.00

Can i use for cancer in vivo mouse experiment?

Asked by: D21017
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

What is the solubility of this product?

Asked by: hawkeye11
Thank you for your question. This chemical, Bafilomycin A1 (CAS 88899-55-2), will dissolve comfortably in DMSO and can be diluted to as low as 20% DMSO in water without problems.
Answered by: Chemical Support 1
Date published: 2017-02-28
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Rated 5 out of 5 by from NIce product from SCBTThis V-ATPase inhibitor worked very well in the macrophages used in our paper. We used it to block macroautophagy. We got the desired results without problems.
Date published: 2018-10-25
Rated 4 out of 5 by from Work well to inhibit autophagyUsed in HeLa cells to inhibit autophagosome and lysosome fusion. Western Blot detected significantly increased accumulation of LC3.
Date published: 2017-12-30
Rated 5 out of 5 by from Great antibody theses reagents worked wellGreat antibody theses reagents worked well
Date published: 2015-10-27
Rated 5 out of 5 by from No commentNo comment
Date published: 2015-09-16
Rated 5 out of 5 by from Worked well in our experimentsWorked well in our experiments, used this in plant cells.
Date published: 2015-09-14
Rated 5 out of 5 by from It works as I expectedIt works as I expected
Date published: 2015-04-10
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Bafilomycin A1 is rated 4.8 out of 5 by 6.
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