BAAT Antibody (ZA-18): sc-100475

BAAT Antibody (ZA-18) is a mouse monoclonal IgG1 kappa light chain antibody that detects the BAAT protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-BAAT antibody (ZA-18) is available as the non-conjugated format. BAAT, or bile acid coenzyme A (CoA): amino acid N-acyltransferase, is a crucial enzyme in bile acid metabolism, specifically responsible for catalyzing the second step in the conjugation of bile acids to taurine or glycine. This process is vital for the efficient excretion of bile acids into bile, which helps prevent the toxic accumulation of unconjugated bile acids in the body. The first step of this reaction involves the conversion of bile acids to CoA thioesters by ACSVL6, a bile acid CoA ligase. BAAT is predominantly localized in the cytoplasm and peroxisomes, with significant expression in the liver, pancreas, intestine, and gallbladder mucosa. Notably, mutations in the gene encoding BAAT have been linked to familial hypercholanemia (FHCA), a condition characterized by fat malabsorption, elevated serum bile acid levels, and associated itching. BAAT (ZA-18) antibody effectively detects this protein, making BAAT monoclonal antibody (ZA-18) an essential tool for research into bile acid metabolism and related disorders.

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BAAT Antibody (ZA-18) References:

1. Complex inheritance of familial hypercholanemia with associated mutations in TJP2 and BAAT.  |  Carlton, VE., et al. 2003. Nat Genet. 34: 91-6. PMID: 12704386
2. The human bile acid-CoA:amino acid N-acyltransferase functions in the conjugation of fatty acids to glycine.  |  O'Byrne, J., et al. 2003. J Biol Chem. 278: 34237-44. PMID: 12810727
3. Differential regulation of cytosolic and peroxisomal bile acid amidation by PPAR alpha activation favors the formation of unconjugated bile acids.  |  Solaas, K., et al. 2004. J Lipid Res. 45: 1051-60. PMID: 15026425
4. Molecular basis of intrahepatic cholestasis.  |  Carlton, VE., et al. 2004. Ann Med. 36: 606-17. PMID: 15768832
5. Bile acid coenzyme A: amino acid N-acyltransferase in the amino acid conjugation of bile acids.  |  Shonsey, EM., et al. 2005. Methods Enzymol. 400: 374-94. PMID: 16399361
6. Human and rat bile acid-CoA:amino acid N-acyltransferase are liver-specific peroxisomal enzymes: implications for intracellular bile salt transport.  |  Pellicoro, A., et al. 2007. Hepatology. 45: 340-8. PMID: 17256745
7. Quantification and regulation of the subcellular distribution of bile acid coenzyme A:amino acid N-acyltransferase activity in rat liver.  |  Styles, NA., et al. 2007. J Lipid Res. 48: 1305-15. PMID: 17379925
8. Genetic polymorphism of bile acid CoA: amino acid N-acyltransferase in Japanese individuals.  |  Tougou, K., et al. 2007. Drug Metab Pharmacokinet. 22: 125-8. PMID: 17495420
9. Inactivation of human liver bile acid CoA:amino acid N-acyltransferase by the electrophilic lipid, 4-hydroxynonenal.  |  Shonsey, EM., et al. 2008. J Lipid Res. 49: 282-94. PMID: 17965457