Azilsartan-d5 (CAS 1346599-45-8)

Azilsartan-d5, indeed a compound of great utility in analytical chemistry. This isotopically labeled form of Azilsartan serves as an invaluable internal standard for the accurate measurement of Azilsartan using gas chromatography (GC) or liquid chromatography (LC) coupled with mass spectrometry (MS) techniques. Azilsartan, known for its role as an antagonist of the angiotensin II type 1 receptor (AT1), exhibits a significant inhibitory effect with an IC50 value of 0.42 µM. It serves as the active metabolite derived from azilsartan medoxomil, a prodrug. Following hydrolysis in the gastrointestinal tract and liver, azilsartan is formed. Notably, Azilsartan acts as an inverse agonist, effectively impeding the angiotensin II-induced accumulation of inositol-1-phosphate in COS-7 cells expressing recombinant human AT1. The use of Azilsartan-d5 as an internal standard allows for precise quantification of Azilsartan, facilitating research related to its metabolism, and receptor interactions.