Axin Antibody (AT1A4): sc-517389
- Axin Antibody (AT1A4) is a mouse monoclonal IgG2b κ provided at 100 µg/ml
- raised against a recombinant protein corresponding to amino acids 546-752 of Axin of human origin
- recommended for detection of Axin of human origin by WB, IP, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Axin Antibody (AT1A4). This work is in progress.
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Axin Antibody (AT1A4) is a mouse monoclonal IgG2b antibody that detects Axin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Axin plays a crucial role in the regulation of the Wnt signaling pathway, acting as a scaffold that facilitates the formation of a complex with β-catenin, adenomatous polyposis coli (APC), and glycogen synthase kinase 3 beta (GSK-3β). This complex is essential for the targeted degradation of β-catenin, thereby preventing its accumulation and subsequent activation of Wnt target genes. The importance of Axin lies in maintaining cellular homeostasis and preventing aberrant signaling that can lead to various diseases, including cancer. Additionally, Axin′s structural similarity to Conductin, which shares 45% identity, suggests overlapping functions in Wnt signaling regulation. Anti-Axin antibody (AT1A4) enables researchers to gain insights into the dynamics of Wnt signaling and its implications in developmental processes and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Axin Antibody (AT1A4) References:
- Roles of Axin in the Wnt signalling pathway. | Kikuchi, A. 1999. Cell Signal. 11: 777-88. PMID: 10617280
- Axin and hepatocellular carcinomas. | Clevers, H. 2000. Nat Genet. 24: 206-8. PMID: 10700164
- Toggling a conformational switch in Wnt/β-catenin signaling: regulation of Axin phosphorylation. The phosphorylation state of Axin controls its scaffold function in two Wnt pathway protein complexes. | Tacchelly-Benites, O., et al. 2013. Bioessays. 35: 1063-70. PMID: 24105937
- Axin Regulates Dendritic Spine Morphogenesis through Cdc42-Dependent Signaling. | Chen, Y., et al. 2015. PLoS One. 10: e0133115. PMID: 26204446
- Axin is expressed in mitochondria and suppresses mitochondrial ATP synthesis in HeLa cells. | Shin, JH., et al. 2016. Exp Cell Res. 340: 12-21. PMID: 26704260
- Axin-1 Regulates Meiotic Spindle Organization in Mouse Oocytes. | He, XQ., et al. 2016. PLoS One. 11: e0157197. PMID: 27284927
- AXIN Shapes Tankyrase ARChitecture. | Guettler, S. 2016. Structure. 24: 1625-1627. PMID: 27705813
- Axin 1 knockdown inhibits osteoblastic apoptosis induced by Porphyromonas gingivalis lipopolysaccharide. | Zhang, K., et al. 2020. Arch Oral Biol. 112: 104667. PMID: 32092441
- Phase separation of Axin organizes the β-catenin destruction complex. | Nong, J., et al. 2021. J Cell Biol. 220: PMID: 33651074
- The Axin scaffold protects the kinase GSK3β from cross-pathway inhibition. | Gavagan, M., et al. 2023. Elife. 12: PMID: 37548359
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Axin Antibody (AT1A4) | sc-517389 | 100 µg/ml | $322.00 |