Avidin D-HRP Avidin-D is a deglycosylated avidin purified from egg white preferred for it's highly specific binding to Biotin

Avidin D-HRP: sc-516217

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Datasheets

    • 15 ml; sufficient for 150 slides
    • Pre-diluted and ready-to-use solution of Avidin-D conjugated to horseradish peroxidase (HRP)
    • Avidin-D is a deglycosylated avidin purified from egg white preferred for it's highly specific binding to Biotin
    • Recommended when using the Labeled Steptavidin Biotin (LSAB) staining method for immunoperoxidase staining of tissues (IHC) or cells (ICC)
    • Recommended for use with conventional species-specific biotinylated secondary antibodies
    • Store at 4° C
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Can you use this for direct detection of biotinylated protein on Western Blot? Any recommendation of what dilution to start with?

Asked by: LizB
Thank you for your question. We have not tested this Avidin D-HRP for this application. This is provided as 15 ml of pre-diluted and ready-to-use solution of Avidin-D conjugated to horseradish peroxidase (HRP). We do not have any recommendation for how much to use for that application. This is something that would have to be determined experimentally.
Answered by: Technical Support
Date published: 2019-04-23
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