AUP1 Double Nickase Plasmid (h): sc-410699-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AUP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AUP1 Double Nickase Plasmid (h) and AUP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AUP1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AUP1 Double Nickase Plasmid (h) | sc-410699-NIC | 20 µg | $410.00 |
AUP1 (ancient ubiquitous protein 1) is an endoplasmic reticulum–associated protein implicated in lipid droplet biology and protein quality control. It participates in ER-associated degradation (ERAD) by linking ubiquitinated substrates to downstream processing machinery, thereby influencing proteostasis and cellular stress responses. Through its roles in ubiquitin signaling and lipid metabolism, AUP1 contributes to pathways governing membrane homeostasis and metabolic adaptation. Dysregulation of these processes is relevant to conditions characterized by altered ER stress handling and lipid storage, including cancer cell metabolic reprogramming and neurodegenerative proteinopathy models.
AUP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AUP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AUP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AUP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AUP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.