Atto 610 N-succinimidyl ester

Atto 610 N-succinimidyl ester, also known as Atto 610 NHS ester, is a fluorescent dye used for labeling biomolecules, such as proteins and nucleic acids, in various biological and biochemical applications. Atto 610 NHS ester belongs to the class of cyanine dyes and is used as a red-fluorescent probe in fluorescence microscopy, flow cytometry, and other imaging techniques. The dye contains an N-hydroxysuccinimide (NHS) ester functional group, which reacts with primary amines (-NH2) of biomolecules, forming stable amide bonds. This reaction is referred to as NHS ester chemistry or amine labeling. When Atto 610 NHS ester reacts with a primary amine, such as the amino group of a protein or nucleic acid, it forms a covalent bond, resulting in the attachment of the dye molecule to the biomolecule of interest. This covalent bond ensures stable and specific labeling of the target molecule. The excitation and emission wavelengths of Atto 610 NHS ester are around 595 nm and 635 nm, respectively, making it suitable for red fluorescence detection in various imaging systems. It is important to note that proper handling and storage conditions should be followed to maintain the stability and functionality of Atto 610 NHS ester. It is recommended to protect the dye from light, moisture, and excessive heat.