Atto 590 NHS ester (CAS 670269-33-7)

Atto 590 NHS ester is a fluorescent labeling compound that is used in molecular biology and bioconjugation research. Its high fluorescence intensity and photostability make it an ideal tool for the study of biological molecules, particularly proteins and nucleic acids. Researchers utilize Atto 590 NHS ester for its ability to form stable amide bonds with primary amines, which are frequently found on the side chains of lysine residues or at the N-termini of proteins. This selective reactivity is leveraged in fluorescence microscopy and flow cytometry to visualize the location and movement of labeled molecules within cells or in more complex biological systems. Additionally, the compound is involved in research aimed at developing new diagnostic assays where the detection of specific biomolecules is necessary, and its bright fluorescence can significantly enhance assay sensitivity. Due to its long-wavelength emission, Atto 590 NHS ester is also used in the study of Förster Resonance Energy Transfer (FRET) experiments, thereby contributing to the understanding of molecular interactions and dynamics.