ATPIF1 Antibody (A-3): sc-271614

ATPIF1 Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects ATPIF1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-ATPIF1 antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ATPIF1, also known as ATPase inhibitory factor 1, plays a crucial role in regulating mitochondrial ATP synthase activity, particularly under low pH conditions. By binding to the C-terminal region of a β subunit of the F1-ATPase, ATPIF1 inhibits the enzyme′s activity, thereby preventing excessive ATP production during metabolic stress. This regulation is vital for maintaining cellular energy homeostasis and protecting cells from potential damage caused by overactive ATP synthesis. Additionally, ATPIF1′s reversible binding to the F1F0-ATPase complex occurs on the surface of endothelial cells, highlighting its importance in vascular function and response to metabolic changes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ATPIF1 Antibody (A-3) References:

1. Nucleotide sequence of cDNA coding the mitochondrial precursor protein of the ATPase inhibitor from humans.  |  Ichikawa, N., et al. 1999. Biosci Biotechnol Biochem. 63: 2225-7. PMID: 10664857
2. The structure of bovine IF(1), the regulatory subunit of mitochondrial F-ATPase.  |  Cabezón, E., et al. 2001. EMBO J. 20: 6990-6. PMID: 11742976
3. An Inhibitor of the F1 subunit of ATP synthase (IF1) modulates the activity of angiostatin on the endothelial cell surface.  |  Burwick, NR., et al. 2005. J Biol Chem. 280: 1740-5. PMID: 15528193
4. The inhibitor protein of the F1F0-ATP synthase is associated to the external surface of endothelial cells.  |  Cortés-Hernández, P., et al. 2005. Biochem Biophys Res Commun. 330: 844-9. PMID: 15809073
5. Identification of a conserved calmodulin-binding motif in the sequence of F0F1 ATPsynthase inhibitor protein.  |  Contessi, S., et al. 2005. J Bioenerg Biomembr. 37: 317-26. PMID: 16341776
6. Supercomplexes and subcomplexes of mitochondrial oxidative phosphorylation.  |  Wittig, I., et al. 2006. Biochim Biophys Acta. 1757: 1066-72. PMID: 16782043
7. The inhibitor protein (IF1) promotes dimerization of the mitochondrial F1F0-ATP synthase.  |  García, JJ., et al. 2006. Biochemistry. 45: 12695-703. PMID: 17042487
8. Inhibitors of the catalytic domain of mitochondrial ATP synthase.  |  Gledhill, JR. and Walker, JE. 2006. Biochem Soc Trans. 34: 989-92. PMID: 17052243
9. Suppression of mitochondrial ATPase inhibitor protein (IF1) in the liver of late septic rats.  |  Huang, LJ., et al. 2007. Biochim Biophys Acta. 1767: 888-96. PMID: 17490602
10. Upregulation of mitochondrial ATPase inhibitory factor 1 (ATPIF1) mediates increased glycolysis in mouse hearts.  |  Zhou, B., et al. 2022. J Clin Invest. 132: PMID: 35575090
11. scRNA-seq reveals ATPIF1 activity in control of T cell antitumor activity.  |  Zhong, G., et al. 2022. Oncoimmunology. 11: 2114740. PMID: 36016697
12. Enhanced glycolysis by ATPIF1 gene inactivation increased the anti-bacterial activities of neutrophils through induction of ROS and lactic acid.  |  Zhong, G., et al. 2023. Biochim Biophys Acta Mol Basis Dis. 1869: 166820. PMID: 37558010

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATPIF1 Antibody (A-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ATPIF1 Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATPIF1 Antibody (A-3) Specifications

Alternate Product Name: ATPIF1

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-106

Epitope Target: ATPIF1

Epitope Species: human

Product Reactivity/Detection: ATPIF1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ATPIF1 Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin